TAILIEUCHUNG - Phylogenetic relationships between Malcolmia, Strigosella, Zuvanda, and some closely related genera (Brassicaceae) from Turkey revealed by inter-simple sequence repeat amplification

The genus Malcolmia is taxonomically problematic, and some of its species have recently beentransferred to the genera Strigosella Boiss. and Zuvanda Dvorák. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. | Research Article Turk J Bot 35 (2011) 17-23 © TÜBİTAK doi: Phylogenetic relationships between Malcolmia, Strigosella, Zuvanda, and some closely related genera (Brassicaceae) from Turkey revealed by inter-simple sequence repeat amplification Bekir DOĞAN1,*, Murat ÜNAL2, Fevzi ÖZGÖKÇE3, Esra MARTİN4, Ayla KAYA5 1Selçuk University, Education Faculty, Department of Science Education, Konya - TURKEY 2Yüzüncü Yıl University, Education Faculty, Department of Biology Education, Van - TURKEY 3Yüzüncü Yıl University, Faculty of Science and Arts, Department of Biology, Van - TURKEY 4Selçuk University, Education Faculty, Department of Biology, 42090 Konya - TURKEY 5Anadolu University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Eskişehir - TURKEY Received: Accepted: Abstract: The genus Malcolmia is taxonomically problematic, and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorák. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. Thus taxonomic problems of species are resolved through DNA-based molecular analyses, which are not affected by environmental factors compared to phenotypic studies. In the present study, the amplifications of the DNA fragments were carried out using ISSR primers, and the phylogenetic relationship among the taxa was revealed through a dendrogram produced as the outcome of the NTSYSpc software. The infrageneric and intergeneric phylogenetic relationships among Malcolmia and other related genera are determined. A very close relationship was determined between Malcolmia chia and M. flexuosa. Strigosella and Zuvanda species were determined to be phylogenetically different from these 2 species. The phylogenetic relationships among the

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