TAILIEUCHUNG - Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers

The present study has investigated the efficiency of regrowth of Bacopa monnieri (L.) Pennell after 6 months of storage along with its clonal fidelity checking. Shoot tips and nodal segments of in vitro grown plantlets were encapsulated and stored at different temperatures and the highest regeneration was achieved by storing shoot tips at 4 °C for a period of 6 months. | Turkish Journal of Botany Research Article Turk J Bot (2013) 37: 1008-1017 © TÜBİTAK doi: Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers Joe Virgin Largia MUTHIAH, Karutha Pandian SHUNMUGIAH, Ramesh MANIKANDAN* Department of Biotechnology, Alagappa University, Karaikudi, India Received: Accepted: Published Online: Printed: Abstract: The present study has investigated the efficiency of regrowth of Bacopa monnieri (L.) Pennell after 6 months of storage along with its clonal fidelity checking. Shoot tips and nodal segments of in vitro grown plantlets were encapsulated and stored at different temperatures and the highest regeneration was achieved by storing shoot tips at 4 °C for a period of 6 months. The response of shoot tips was found to be much better when compared to nodal segments in the case of forming healthy shoots and roots. The genetic stability of regrown plantlets was analysed by using RAPD and ISSR markers. Twenty ISSR primers produced 130 clear and reproducible amplicons, of which 125 bands were monomorphic. A total of 115 bands were produced by 25 RAPD markers with 94% monomorphism. This corresponds to a very low genetic variation pertaining to the clonal nature of the regrown plantlets with that of the mother plant. By means of HPLC biochemical analysis, bacoside A content in the regrown plants was also found to be comparable with that of the mother plant. Therefore, the protocol derived can be efficiently utilised for storing the encapsulated shoot tips of B. monnieri for about 6 months. Key words: Encapsulation, storage, regrowth, genetic fidelity, ISSR, RAPD 1. Introduction Plants have been an important source of medicine as they synthesise and preserve a variety of biochemical products. The widespread reliance on herbal remedies in almost all parts of

• Nông nghiệp
• Genetic fidelity
• Encapsulated in vitro tissues
• Bacopa monnieri L
• Inter simple sequence repeat
• Random amplified polymorphic DNA
• Musa spp
• Somaclonal variation
• ISSR and genetic fidelity
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• Genetic fidelity analysis
• Different cultivars of gerbera
• Gerbera (Gerbera jamesonii Bolus)
• DNA based molecular marker RAPD
• ISSR marker
• Plants by ISSR markers
• Micropropagated gerbera (Gerbera jamesonii Bolus)
• Genetic fidelity of micropropagated gerbera
• Clonal fidelity
• Assessment of clonal fidelity
• Genetic analysis
• Tissue culture raised banana
• Banana (Musa spp ) plant
• Viola patrinii
• Leaf explants
• Micropropagated plants
• Random amplified polymorphic DNA
• Vanda orchids
• Genetic fidelity and ISSR primers
• ISSR primers
• Micropropagated plantlets
• Vanda hybrid ‘Dr
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• In vitro
• Nodal explants
• ISSR markers
• Assessment of genetic fidelity
• Acclimatized plantlets
• LA Lilium
• SSR marker
• In vitro regenerated plants
• In vitro regenerates
• Abiotic Stress
• SRO Family
• Characterization
• Plant Antioxidative
• Heavy Metal Toxicity
• Genetic and Molecular
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• Growth regulators
• In vitro regeneration
• RAPD markers
• gladiolus (Gladiolus hybrida L )
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• Direct embryogenesis
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