TAILIEUCHUNG - In vitro plant regeneration via petiole callus of Viola patrinii and genetic fidelity assessment using RAPD markers

The RAPD profile of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. This protocol could be successfully used for the mass multiplication and germplasm conservation of this medicinal plant. | Research Article Turk J Bot 36 (2012): 358-368 © TÜBİTAK doi: In vitro plant regeneration via petiole callus of Viola patrinii and genetic fidelity assessment using RAPD markers Gururaj CHALAGERI*, Uddagiri Venkanna BABU Plant Tissue Culture Lab, Research & Development Centre, The Himalaya Drug Company, Makali, Bangalore 562 123 - INDIA Received: Accepted: Abstract: Viola patrinii DC. (Violaceae) petiole explants were used for inducing calli. Significant callus proliferation was observed on MS medium supplemented with μM NAA and μM BA. Shoot regeneration was achieved upon transferring the green friable calli to MS medium with a 2-fold dilution of potassium dihydrogen phosphate supplemented with μM Kn and μM NAA. Maximum shoot regeneration was achieved in 4 weeks. Multiple shoots were separated and further cultured in half-strength MS medium supplemented with μM IBA with 2% sucrose. Profuse root development was observed after 20-25 days of culturing. Regenerated plants were successfully transferred to soil, showing an 85% survival rate. The genetic fidelity of the micropropagated plants was analysed using random amplified polymorphic DNA (RAPD) markers. The RAPD profile of the regenerated plants showed similar banding patterns to that of the mother plant thus demonstrating the homogeneity of the micropropagated plants. This protocol could be successfully used for the mass multiplication and germplasm conservation of this medicinal plant. Key words: Viola patrinii, regeneration, micropropagation, leaf explants, RAPD, genetic fidelity Introduction Viola patrinii DC. (Violaceae), commonly known as China violet, is an important medicinal herb, mentioned in traditional medicine for a variety of therapeutic applications including the purification of blood and the treatment of bruises and ulcers (Kirtikar & Basu, 1995); in the Chinese system of medicine it is recommended for use against .

• Nông nghiệp
• Viola patrinii
• Leaf explants
• Genetic fidelity
• Micropropagated plants
• Random amplified polymorphic DNA
• Identification of genetic polymorphism
• Polymerase chain reaction
• Molecular biology techniques
• Sequence characterised amplified regions
• DNA Isolation
• RAPD primers
• Heat tolerance chickpea
• Molecular characterization
• Cicer arietinum L
• Sex discrimination
• Nile tilapia fish
• Oreochromis niloticus
• Genetical sex
• Genetic diversity
• Molecular marker
• Random amplified polymorphic DNA fingerprinting
• Pleurotus strains
• Genetic diversity characterization
• Sodium nitrite
• Assessment of genetic polymorphism
• Genetic polymorphism
• Sugarcane somaclones
• Genomic template stability
• Tolerance index
• Hordeum vulgare L
• Genetic relationship
• HPV 16
• HPV 18
• Cervical cancer patient
• Human papilloma virus isolated
• Turkish Journal of Botany
• Tạp chí Thực vật học
• Long terminal repeat retrotransposons
• DNA methylation status
• Coupled restriction enzyme digestion random amplification
• Cephalaria Schrad
• Cephalaria sumbuliana
• Cephalaria davisiana
• Related taxa
• Amplified fragment length polymorphisms
• Molecular marker systems
• Sequence related amplified polymorphism
• Unweighted pair group method with arithmetic averages
• Sugar beet
• Molecular markers
• Polymorphic primers
• DNA markers
• RAPD markers
• Genetic linkage mapping
• Poncirus trifoliata L
• C
• reticulata Blanco
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• Đề tài nghiên cứu tế bào gốc
• Tiểu luận công nghệ sinh học
• Đề tài kỹ thuật Rapd
• Báo cáo thực hành hóa sinh
• Luận văn nghiên cứu đa dạng sinh học
• Tiểu luận Kỹ thuật di truyền
• Cotton Gossypium hirsutum L
• Polymerase chain reaction method
• Gossypium genotypes
• Genetic relationships
• Velezia L
• Unweighted pair group method
• RAPD technique
• Plant genetic resources
• RAPD dendrogram
• Phenotypic similarity
• Terrestrial orchids
• Principal component analysis
• Principal coordinate analysis
• Natural source
• Primer combinations
• Green gram genotypes
• Morphological markers
• Simple sequence repeat
• Tạp chí khoa học
• Mối quan hệ di truyền
• Kỹ thuật RAPD
• Quan hệ di truyền
• Đa dạng di truyền
• Giống đậu tương
• DNA đa hình
• Random Amplified Polymorphic
• Nhân bản ngẫu nhiên
• Hệ số tương đồng di truyền
• Chickpea Cicer arietinum L
• Root rot
• Pathogen free seed
• Genetic diversity analysis of citrus cultivars
• Bellis perennis
• RAPD PCR analysis
• Genomic structures of species
• Rhaponticoides iconiensis
• Oxytona Bernh
• Papaver accessions
• Oxytona species
• Polymerase chain reactions