TAILIEUCHUNG - Characteristics of the cystatin gene in some Vietnamese mungbean cultivars (Vigna radiata (l.) willzek)

Comparision of the amino acid sequences of genes encoding cystatin in mungbean cultivars (KP11, KPS1, MN93, 263) with one mungbean cultivar on NCBI (AF454396), results shows that amino acid sequences was 100% homologous. The result obtained suggests that need further research on the role of control active of intron regions When the mungbean cultivars live In drought conditions. | Nguyễn Vũ Thanh Thanh và cs Tạp chí KHOA HỌC & CÔNG NGHỆ 78(02): 79 - 86 CHARACTERISTICS OF THE CYSTATIN GENE IN SOME VIETNAMESE MUNGBEAN CULTIVARS (Vigna radiata (L.) Willzek) Nguyen Vu Thanh Thanh1, Chu Hoang Mau2* 1 2 Department of Genetics, Faculty of Life Science, College of Science-TNU Department of Genetics and Modern Biology, Faculty of Bilogy and Agro-Technic, College of Education-TNU SUMMARY Cystatins are inhibitors of cystein proteases belonging to papain family. In plants, cystatins are found to be involved in drought stress. This paper our finding about the genes encoding cystatins isolated from Vietnam mungbean cultivars (Vigna radiata L. Wilczek) with different level of drought tolerance. Genomic DNA of four mungbean cultivars, including two with high drought tolerance (KP11 and KPS1) and two with low drought tolerance (MN93, 263) are isolation for gene cloning using cystatin specific primers. Sequencing data of the four cloned cystatin gene fragments proved to be that of cystatin gene with a length of 1115 nucleotides, deviding into 2 exons and 1 intron. The PRF of coding regions of cystatin gene comprises of 267 nucleotides with encoding a polypeptide of 88 amino acids. The nucleotide sequences of the four cultivars show variations in different sites although the amino acid sequences do not differ each to other and even to that announced under the code AF454396. Possible regulatory differences are discussed in relation to the variation found in intron region. Nucleotide sequence of cystatin gene in the four mungbean cultivars (KP11, KPS1, MN93 and 263) have a high level of similarity ( - ), without a change in the 2 exons, but there are 6 the single nucleotide polymorphism located in the introns. Comparision of the amino acid sequences of genes encoding cystatin in mungbean cultivars (KP11, KPS1, MN93, 263) with one mungbean cultivar on NCBI (AF454396), results shows that amino acid sequences was 100% homologous. The result obtained

• Sinh học
• Characteristics of the cystatin gene
• Some Vietnamese mungbean cultivars
• The mungbean cultivars live
• Vietnam mungbean cultivars
• Mungbean cultivar on NCBI
• In drought conditions
• Wax morphology
• Unfavourable growth conditions
• Cuticular wax morphology
• Flag leaves
• Drought sensitive
• Drought tolerant wheat
• Wheat (Triticum aestivum L
• Effect of nitrogen application
• Dry matter production
• Nitrogen application levels on growth of rice
• Growth of rice under drought stress conditions
• Drought stress conditions
• Hydro climatological drought conditions
• Using high resolution model simulation
• Coupled WEHY HCM model
• Standardized precipitation index
• Streamflow drought index
• Agricultural science
• Generation mean analysis in wheat
• Drought stress
• Gene action
• Scaling test
• Heterosis studies Various morphological traits
• Various morphological traits of rice
• Rice under drought conditions
• Water stress conditions
• Additive gene action
• Fatty acids
• Wax esters
• Growth and development
• Quality of wax esters
• Alcohol and cuticle formation
• Drought sensitive wheats
• Wheats (Triticum aestivum)
• drought conditions
• earth sciences
• Research
• scientific research
• scientific reports
• Inbred lines
• Stay green
• CMS sources
• Texas 16 R
• Drought conditions (Helianthus annuus L
• Normal moisture condition
• Path coefficient analysis
• Season drought conditions
• Yield and yield component traits
• Advanced breeding lines
• Phenotypic diversity
• Cluster analysis
• Pod yield
• Rainfed conditions
• Drought tolerance related traits
• Phenotypic divergence for yield
• Seedling parameters
• Multivariate analysis of sesame genotypes
• Induced drought conditions
• Multivariate analysis
• Sesame genotypes
• Lipid peroxidation
• Susceptible genotypes on tomato
• In vitro evaluation of physiological
• Biochemical changes
• Genotypes on tomato
• Glycine max
• water stress
• P5CS genes
• PAP3 genes
• BZIP genes
• Drought stress climatic conditions
• Oryza sativa L
• Water deficit conditions
• Different genotypes of rice
• Yield attributes under well watered
• Supplementation induced influences
• Split plot design
• Water stress tolerance in field conditions
• Field conditions
• Inbred lines of maize
• Flavonoid dynamic responses
• Arabidopsis thaliana
• Flavonoid biosynthesis
• Plant bacteria symbiosis
• Antioxidant enzyme
• Brassica napus
• Gene expression
• Physiological traits
• Agronomic traits
• Grain yield
• Photosynthetic efficiency
• Stability index
• Winter wheat cultivar
• Drought tolerance
• Transgenic plant
• Isopentenyl transferase
• CKs biosynthesis
• GmIPT10 overexpressing transgenic soybean
• Optimal conditions
• SNP markers
• Applying SNP markers
• Breeding drought tolerant maize hybrids
• Maize hybrids
• Journal of Botany
• Abiotic stress
• Forage crop
• Transcription regulation
• Growth parameters
• Turkish Journal of Agriculture and Forestry
• Tạp chí Nông Lâm nghiệp
• Corn hybrids grain protein
• Prolonged stress conditions
• Preserving nitrogen source
• Chickpea Cicer arietinum L
• Chickpea lines
• Non stress environments
• Semi arid tropics
• Nitrogen shortage
• Seed quality
• Peanut genotypes
• Poor nitrogen conditions
• Yielding peanut lines
• Dau Giay cultivar
• Genetic diversity
• Genetic divergence of maize genotypes