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Comparision of the amino acid sequences of genes encoding cystatin in mungbean cultivars (KP11, KPS1, MN93, 263) with one mungbean cultivar on NCBI (AF454396), results shows that amino acid sequences was 100% homologous. The result obtained suggests that need further research on the role of control active of intron regions When the mungbean cultivars live In drought conditions. | Nguyễn Vũ Thanh Thanh và cs Tạp chí KHOA HỌC & CÔNG NGHỆ 78(02): 79 - 86 CHARACTERISTICS OF THE CYSTATIN GENE IN SOME VIETNAMESE MUNGBEAN CULTIVARS (Vigna radiata (L.) Willzek) Nguyen Vu Thanh Thanh1, Chu Hoang Mau2* 1 2 Department of Genetics, Faculty of Life Science, College of Science-TNU Department of Genetics and Modern Biology, Faculty of Bilogy and Agro-Technic, College of Education-TNU SUMMARY Cystatins are inhibitors of cystein proteases belonging to papain family. In plants, cystatins are found to be involved in drought stress. This paper our finding about the genes encoding cystatins isolated from Vietnam mungbean cultivars (Vigna radiata L. Wilczek) with different level of drought tolerance. Genomic DNA of four mungbean cultivars, including two with high drought tolerance (KP11 and KPS1) and two with low drought tolerance (MN93, 263) are isolation for gene cloning using cystatin specific primers. Sequencing data of the four cloned cystatin gene fragments proved to be that of cystatin gene with a length of 1115 nucleotides, deviding into 2 exons and 1 intron. The PRF of coding regions of cystatin gene comprises of 267 nucleotides with encoding a polypeptide of 88 amino acids. The nucleotide sequences of the four cultivars show variations in different sites although the amino acid sequences do not differ each to other and even to that announced under the code AF454396. Possible regulatory differences are discussed in relation to the variation found in intron region. Nucleotide sequence of cystatin gene in the four mungbean cultivars (KP11, KPS1, MN93 and 263) have a high level of similarity (99.6% - 99.8%), without a change in the 2 exons, but there are 6 the single nucleotide polymorphism located in the introns. Comparision of the amino acid sequences of genes encoding cystatin in mungbean cultivars (KP11, KPS1, MN93, 263) with one mungbean cultivar on NCBI (AF454396), results shows that amino acid sequences was 100% homologous. The result obtained
