TAILIEUCHUNG - Electrofusion of Saccharomyces cerevisiae auxotrophic mutants of identical mating type using a laboratory made system

Yeast protoplasts from haploid auxotroph strains DC6 (MATα) and FY73 (MATα) were exposed to an inhomogeneous alternating field (AC 200 V/cm). Due to the dielectrophoretic aggregation two or more cells with close membrane contact were formed between the two electrodes. | Turk J Biol 27 (2003) 1-5 © TÜB‹TAK Electrofusion of Saccharomyces cerevisiae Auxotrophic Mutants of Identical Mating Type Using a Laboratory-Made System Gökhan CORAL* Department of Biology, Faculty of Arts and Sciences, Mersin University, 33342 Çiftliköy-Mersin - TURKEY Ömer ÇOLAK Department of Biology, Faculty of Arts and Sciences, Çukurova University, 01330 Balcal›-Adana - TURKEY M. Nisa ÜNALDI Department of Biology, Faculty of Arts and Sciences, Mustafa Kemal University, 31040 Hatay - TURKEY Burhan ARIKAN Department of Biology, Faculty of Arts and Sciences, Çukurova University, 01330 Balcal›-Adana - TURKEY Received: Abstract: Yeast protoplasts from haploid auxotroph strains DC6 (MATα) and FY73 (MATα) were exposed to an inhomogeneous alternating field (AC 200 V/cm). Due to the dielectrophoretic aggregation two or more cells with close membrane contact were formed between the two electrodes. The effects of different DC pulses were investigated to determine critical fusogenic pulse strength. In the results, the maximum fusant cells were obtained only when 8 kV field strengths were applied. These findings showed that the optimum fusogenic pulse strength for yeast protoplasts is 8 kV/cm under our experimental conditions. After fusion, the DNA contents of both hybrid and individual parental strains were compared. The amount of DNA in the hybrid was found to be about twofold higher than that in the individual parental strain. Key Words: Electrofusion, Saccharomyces cerevisiae ‹dentik Eflleflme Tipine Sahip Saccharomyces cerevisiae Oksotrofik Mutantlar›n›n Laboratuvar Yap›m› Bir Sistem Kullan›larak Elektrofüzyonu Özet: Bu çal›flmada DC6 (MATα) ve FY73 (MATα) haploid oksotrof maya sufllar›ndan elde edilen protoplastlar, homojen olmayan bir alternatif alana (200 V/cm AC) maruz b›rak›ld›. Dielektroforetik agregasyona ba¤l› olarak, iki elektrot aras›nda kalan iki ya da daha fazla say›daki hücrede yak›n membran temas› oluflturuldu. Hücreleri füzyona .

• Sinh học
• Saccharomyces cerevisiae
• Yeast protoplasts
• Auxotroph strains DC6
• Inhomogeneous alternating
• Polyethylene glycol
• RGT saccharomyces cerevisiae
• Prebiotic MOS
• Isolation and characterization
• Rabbit gut transmitted Saccharomyces cerevisiae
• Lên men rượu vang vải thiều
• Chủng nấm men Saccharomyces Cerevisiae LC3
• Saccharomyces Cerevisiae LC3
• Quá trình lên men rượu vang vải
• Phòng thí nghiệm vi sinh
• Tạp chí Công nghệ Sinh học
• Bài viết về sinh học
• Cải biến chủng
• Chủng nấm men Saccharomyces cerevisiae D8
• Lentinula edodes
• PCRRFLP and SDSPAGE
• Saccharomyces cerevisiae and Lentinula edodes
• Region based diversity studies
• Sử dụng Saccharomyces cerevisiae rv002
• Men rượu vang từ quả sim
• Saccharomyces cerevisiae RV002
• Phần mềm SPSS
• Hiệu quả lên men
• Agricultural science
• Protein enrichment of yam peels
• Fermentation with Saccharomyces cerevisiae
• Proximate composition
• Tạp chí khoa học
• Sàng lọc chủng nấm men Saccharomyces Cerevisiae
• Khả năng tích lũy kẽm cao
• Chủng nấm men
• Chủng nấm men S
• cerevisiae
• Scientific reports
• scientific research
• Structural biochemistry
• decarboxylase
• biochemical studies
• khả năng hấp thu
• kim loại nặng
• nấm men Saccharomyces cerevisiae
• hóa học môi trường
• đề tài nghiên cứu
• nghiên cứu khoa học
• breast cancer
• medicine
• phosphatidate
• medical knowledge
• biochemistry
• Saccharomyces cerevisiae H2 RNase
• cytoplasm
• analysis of cytoplasmic
• báo cáo khoa học
• enzyme amylase
• SẢN XUẤT MEN RƯỢU
• Đồng bằng sông Cửu Long
• α amylase
• β−glucanase
• Bacillus subtilis
• Gene cloning
• Công nghệ sinh học
• Môi trường nhân giống
• Chủng Saccharomyces cerevisiae MS42
• Qúa trình sinh trưởng của nấm men
• Lactobacillus acidophilus
• Simulated gastric juice
• Tạp chí sinh học
• Intergenic sequences
• Sense and antisense sequences
• Nghiên cứu quy trình tách chiết Beta glucan
• Quy trình tách chiết Beta glucan
• Quy trình tách chiế
• Tế bào Saccharomyces Cerevisiae
• Bã men bia
• Osmotic stress
• Oxidative stress
• Transcriptional repression
• Enzyme invertase
• Glucose sensing
• Glucose uptake
• Yeast cells
• Cyclin dependent protein
• Cytoplasmic sensor proteins
• Glucose repression
• Hexose transporters
• Non histone proteins
• HXT4 gene
• Thioredoxin reductase
• Methionine sulfoxide
• TRX2 genes
• Thioredoxin system
• Tạp chí Đại học công nghiệp thực phẩm
• Tinh dầu quế
• Saccharomyces cerevisiae và aspergillus niger
• Phương pháp khuếch tán
• Phương pháp ức chế hệ sợi
• Hexokinase PII
• SUC2 gene
• Gcr2 and Sgc1 mutants
• Ampicillin activates
• CWI pathway
• Candida albicans
• MAP kinase 1