TAILIEUCHUNG - Effect of plant oils on mycelial biomass production, biosynthesis and antioxidants of exopolysaccharide by ophiocordyceps sinensis
Ophiocordyceps sinensis (syn. Cordyceps sinensis) is a well-known entomopathogenic fungus with many significant bioactivities such as antioxidants, immunomodulatory and antitumor, etc. In Vietnam, its mycelial biomass has been cultured artificially in a liquid medium and has been studied application since 2013. | Tạp chí Khoa học công nghệ và Thực phẩm 13 (1) (2017) 3-10 EFFECT OF PLANT OILS ON MYCELIAL BIOMASS PRODUCTION, BIOSYNTHESIS AND ANTIOXIDANTS OF EXOPOLYSACCHARIDE BY OPHIOCORDYCEPS SINENSIS Hang Le Thi Thuy1,5*, Tuyet Nguyen Thi Thu2, Phuong Bach Thi Bich2, Trang Tran Minh2, Thu Huynh3, Hiep Dinh Minh4, Thang Nguyen Tien5 1 Ho Chi Minh City University of Food Industry 2 Ho Chi Minh City University of Science 3 Ho Chi Minh City University of Technology 4 Ho Chi Minh City Agricultural Hi-Tech Park 5 Vietnam Academy of Science and Technology *Email: hangltt@ Received: 23 October 2017; Accepted for publication: 5 December 2017 ABSTRACT Ophiocordyceps sinensis (syn. Cordyceps sinensis) is a well-known entomopathogenic fungus with many significant bioactivities such as antioxidants, immunomodulatory and antitumor, etc. In Vietnam, its mycelial biomass has been cultured artificially in a liquid medium and has been studied application since 2013. Many previous researches demonstrated that exopolysaccharides (EPS) was secreted by the fungus with many integral bioactive activities. Thus, the aim of this study is to enhance the biosynthesis potential of EPS of O. sinensis fungus by implementing sunflower oils and coconut oils at concentrations from 1% to 5% (v/v), and olive oil at concentrations between 1% and 10% in culture medium. The results showed that the mycelial biomass and EPS production of O. sinensis fungus increased considerably compared to non-oil medium. The EPS yields for olive oil (5%), sunflower oil (3%) and coconut oil (4%) were g/L, g/L and g/L, respectively. Moreover, the ABTS (2,2'- azino - bis (3 – ethylbenzothiazoline – 6 - sulphonic acid) radical scavenging activity of EPS extracted from the olive oil-containing medium of 5% rose significantly compared to the control EPS. As a result, the data has demonstrated that the 5% olive oil-containing medium had the ability to boost EPS production of O. sinensis fungus and improve .
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