TAILIEUCHUNG - Assessment of two highly polymorphic barley microsatellite markers for detecting polymorphism in wheat

Simple sequence repeat (SSR) DNA polymorphism is a PCR-based marker system, also known as the microsatellite marker system. SSRs are tandemly repeated 1 to 5 nucleotide units dispersed throughout eukaryotic genomes. | Turk J Biol 26 (2002) 9-12 © TÜB‹TAK Assessment of Two Highly Polymorphic Barley Microsatellite Markers for Detecting Polymorphism in Wheat E. Banu BÜYÜKÜNAL BAL, Mahinur S. AKKAYA Middle East Technical University, Department of Chemistry, TR-06531, Ankara - TURKEY Received: Abstract: Simple sequence repeat (SSR) DNA polymorphism is a PCR-based marker system, also known as the microsatellite marker system. SSRs are tandemly repeated 1 to 5 nucleotide units dispersed throughout eukaryotic genomes. They are considered one of the most powerful molecular markers for many diverse applications in genome related studies, ., DNA fingerprinting for discrimination of genotypes and genetic mapping. Locus specificity, co-dominant inheritance and easy detection with PCR amplifications are some of the favorable features of this marker system. On the other hand, isolation of SSR markers depends on time-and labor-consuming processes. The need to obtain sequence information for locus specific primer design is the major drawback. Therefore, the application of SSR markers in a cross-species manner would be valuable. In this study, two highly polymorphic barley SSR markers were used to test the cross transportability and polymorphism property of barley SSR primers on wheat genotypes. The results indicated that barley specific SSR primers could generate PCR amplification products on the wheat genome in the absence of polymorphism. Key Words: SSR, microsatellite, DNA-based markers, barley and wheat. Bu¤dayda Polimorfizm Saptanmas›nda ‹ki Yüksek Düzeyde Polimorfik Arpa Mikrosatelit Belirleyicisinin Kullan›lm›n›n ‹ncelenmesi Özet: Mikrosatelitler olarak da bilinen BDTD (Basit Dizilim Tekrar DNA)lar› birbiri ard›na tekrarlanan 1 ila 5 nükleotit üniteleri olup, ökaryot genomlar›nda kromozomlar üzerinde da¤›lm›fl olarak bulunurlar. BDTD belirleyicileri en iyi DNA tabanl› belirleyilerden biri olarak kabul edildiklerinden, DNA parmak izi analizi ile genotip farkl›l›klar›n›n

• Sinh học
• DNA based markers
• Barley and wheat
• Simple sequence repeat
• PCR based marker system
• DNA polymorphism
• Eukaryotic genomes
• Genome Biology
• Repeat rich eukaryotic genomes
• Significant challenge in genomics
• High quality assembly
• Microbial genomes increasingly feasible
• Microbial eukaryotes
• Genome quality estimation
• Metagenome assembled genomes
• Biological information
• Lecture Genetics
• Human genetics
• Eukaryotic chromosome
• Structure of eukaryotic chromosomes
• Linear DNA molecules
• Campbells Biology
• Lecture Biology
• Biological sciences
• Biological organization
• Systems biology
• Gene structure
• Unicellular organisms
• Multiple sequence alignments
• Homologous genes
• BMC Genomics
• Genome annotation
• Peptide sequence tag
• Tandem mass spectrometry
• Eukaryotic species
• Transcriptomics data
• Genome sequenced
• Missing chromosomal regions
• Avian genomes
• Non coding regions
• microbial biodiversity
• Genomic approaches
• ecological niche
• microbial infection
• ecological stress
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• Gene regulation in eukaryotes
• Eukaryotic gene regulation
• Gene regulation
• Genetics from genes to genome
• How genes travel on chromosomes
• How genes are regulated
• Beyond the individual gene and genome
• The eukaryotic chromosome
• Gene regulation in prokaryote
• AP Biology
• The study of life
• Chromosomal basis of inheritance
• DNA technology
• Tracing phylogeny
• BMC Bioinformatics
• Next generation sequencing
• Species identification
• Locality sensitive hashing
• Big data
• High throughput sequencing
• Cytosine methylation
• Unmethylated cytosines
• Length preference
• Tandem repeats
• Simple Sequence Repeats
• Cellular processes
• Comparative genomics
• Gene expression
• Natural diversity
• Eukaryotic genes
• Histomonas meleagridis
• Nanopore technology
• Leucine rich repeat protein
• Protozoan parasite
• Accessible transposable element
• Embryo development
• Transposable elements
• Distinct biological functions
• Arabidopsis thaliana
• DNA methylation
• Chromatin modification
• Occurring primarily
• Cytosine nucleotides
• De novo genome assembly
• Assembly reconciliation
• Multiple assemblies
• Horizontal gene transfer
• Human genome
• Phylogenetic trees
• Transposable element
• Eukaryotic genomes analyzed
• Sequencing technology
• Traverse transposable elements
• Topologically associating domains
• Severe developmental disorders
• Three dimensional genome structure
• Genome evolution
• Intense interrogation