TAILIEUCHUNG - Molecular characterization of Borrelia burgdorferi linear plasmids by DNA hybridization, PCR, two dimensional gel electrophoresis, and electron microscopy

Borrelia burgdorferi sensu stricto is the predominant cause of Lyme disease. B. burgdorferi strains carry several linear and circular plasmids and this may be also used as a distinguishing property of the bacteria. The presence of a mixed population of linear and circular plasmids makes separation and characterization of each kind very difficult. | Turk J Biol 31 (2007) 73-80 © TÜB‹TAK Molecular Characterization of Borrelia burgdorferi Linear Plasmids by DNA Hybridization, PCR, Two-Dimensional Gel Electrophoresis, and Electron Microscopy Ali KARAMI, Seyed Mohammad Javad HOSSEYNI, Yaser KIARUDI Research Center of Molecular Biology, Baqiyatallah University of Medical Science. . Box 19945-581, Tehran-IRAN Received: Abstract: Borrelia burgdorferi sensu stricto is the predominant cause of Lyme disease. B. burgdorferi strains carry several linear and circular plasmids and this may be also used as a distinguishing property of the bacteria. The presence of a mixed population of linear and circular plasmids makes separation and characterization of each kind very difficult. In this study, 7 clinical strains of skin and CSF isolates were determined by clonal selection and plasmid profile of B. burgdorferi by electron microscopy and 2-dimensional agarose gel electrophoresis. The results demonstrate that the skin isolate (DK1) has 2 different phenotypes A and B, but the CSF isolate (DK6) has only 1 phenotype. Plasmid profiles from different strains of B. burgdorferi showed extreme complexity analyzed by conventional agarose electrophoresis. In conclusion, the DK1 strain has 10 plasmids in comparison to 6 plasmids in the DK6 strain. This could be due to a mixture of 2 phenotypes in the DK1 strain. Most of the plasmids in both strains were linear. This conclusion is supported by DNA hybridization, electron microscopy of plasmids, and amplification experiments with OspA-B specific primers. Key Words: Borrelia burgdorferi, linear plasmid, plasmid profile, 2-dimensional gel electrophoresis, electron microscopy Introduction Borrelia burgdorferi is the spirochetal agent that causes Lyme borreliosis. Numerous approaches have been used to demonstrate the genetic and phenotypic diversity among Borrelia strains (1). It has been shown that the North American strain Borrelia B31 and the 2 Swedish strains G25 and G74

• Sinh học
• Borrelia burgdorferi
• Linear plasmid
• Plasmid profile
• 2 dimensional gel electrophoresis
• Electron microscopy
• Blacklegged tick
• Ixodes scapularis
• Lyme disease
• Infection prevalence
• Grand River valley
• Polymerase chain reaction
• Kenora District
• BMC Veterinary Research
• Gene ticks
• Lyme Borreliosis
• Tick borne relapsing fever
• Basic membrane protein A
• Murine macrophage cell line
• Lyme arthritis
• Macrophage inflammatory protein 2
• BMC Genomics
• Tick borne diseases
• Tick transmission
• Zinc superoxide dismutase
• tài liệu báo cáo nghiên cứu khoa học
• cách trình bày báo cáo
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• trình bày báo cáo
• tài liệu báo cáo khoa học
• kiến thức bênh thú y
• nghiên cứu bệnh thú y
• BMC Pulmonary Medicine
• Phrenic nerve palsy
• Lyme neuroborreliosis
• Differential diagnoses
• Public health
• Erythema migrans