TAILIEUCHUNG - Expression analysis of genes in the nif cluster of Clostridium beijerinckii

The nif genes of Clostridium beijerinckii NRRL B593 occupy a region of about 16 kilobases. Besides the two glnB-like genes, five other genes are interspersed between the nifNB and the nifVω genes. | Turk J Biol 31 (2007) 7-11 © TÜB‹TAK Expression Analysis of Genes in the Nif Cluster of Clostridium beijerinckii 1,2 Murat KASAP 1 , Jiann-Shin CHEN 1 Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 USA 2 Department of Medical Biology, Kocaeli University Medical Center, 41380 Umuttepe, Kocaeli - TURKEY Received: Abstract: The nif genes of Clostridium beijerinckii NRRL B593 occupy a region of about 16 kilobases. Besides the two glnB-like genes, five other genes are interspersed between the nifNB and the nifVω genes. An expression analysis of the nif genes in nitrogenfixing and non-nitrogen-fixing cells with probes generated from various regions of the nif cluster by northern blot analysis revealed the presence of four different transcripts in nitrogen-fixing cells. Two of these transcripts had the predicted sizes spanning from nifH to nifK and from nifVω to nifVα, respectively. The other two transcripts did not have the expected sizes, but they suggested the presence of two other polycistronic mRNAs consisting of nifE-nifNB and nirJ1-nirJ2-nirD-nirH, respectively. The absence of the nif and nif-associated mRNAs in RNA samples from non-nitrogen-fixing cells indicated that nif-associated genes are regulated in parallel to nif genes. Key Words: Nitrogen fixation, transcriptional analysis, Clostridium beijerinckii, northern blotting, nif cluster Clostridium beijerinckii’da Bulunan Nif Genlerinin Ekspresyon Analizi Özet: Clostridium beijerinckii NRRL B593’de bulunan nif genleri 16 kilobaz çiflik bir bölgeyi kaplamaktad›r. Bilinen iki glnB benzeri genlerin yan›s›ra, befl adet farkl› gen nifNB ve nifVω aras›n› iflgal eder. Bu cal›flmada azot fiksasyonu yapan ve yapmayan iki farkl› C. beijerinckii kültür tipinde nif gen grubundan düzenlenen problarla yap›lan transkripsiyon analizinde dört farkl› transkripsiyonun azot fikse eden kültürlerde varl›¤› northern blot analizi ile gösterildi. Bu .

• Sinh học
• Nitrogen fixation
• Transcriptional analysis
• Clostridium beijerinckii
• Northern blotting
• Nif cluster
• Rhizobial symbiosis
• Nod factor
• Utilization in nitrogen fixation
• Plant genetic control
• Plant genetic control of nodulation
• Carbohydrate polymers
• Residual nitrogen
• Cellulosic material
• Ideal cellulose
• Biological nitrogen fixation
• Soil fertility
• Nitrogen economy
• Environmental terms
• Review on the role
• Cicer arietinum L Mesorhizobium
• Plant dry matter
• Nitrogen and phosphorus content
• Chickpea (Cicer arietinum L )
• Leguminous plants
• Nitrogen status of soybean
• Vegetative growth
• Biochemical mechanisms
• Cicer arietinum
• Symbiotic nitrogen fixation
• Micronutrient deficiency
• Chickpea Cicer arietinum L
• Cicer arietinum L
• N2 fixation
• Antagonistic effect
• Biological control bacteria pseudomonas
• Inhibits free nitrogen fixing bacteria
• Nitrogen fixing bacteria in the rhizosphere
• Glomus mosseae
• Mycorrhizal fungus
• Arbuscular mycorrhizal
• Nitrogen fixing bacteria
• Identification of nitrogenfixing bacteria isolated
• Agricultural soil
• 16S rRNA sequence similarity
• BMC Genomics
• Vulcanococcus limneticus sp nov
• Nitrogenase genes
• Genomic island
• Horizontal gene transfer
• High P soil
• Phosphorus fixation
• P fertility Regime
• Crop in high p soil
• Supplemental enhancement of nitrogen
• BMC Plant Biology
• Endophytic bacteria
• Oxalotrophic bacteria
• Vertical transmission
• Cysteine protease
• Programmed cell death
• Fixing atmospheric nitrogen
• Tripartite symbiotic
• cowpea genotypes
• poverty alleviation
• BNF applications
• sustainable agriculture
• Plant Nutrition
• Macronutrients
• Nutrient Uptake
• Root Nodules
• Nutritional Quality
• Plant growth promoting bacteria
• Indole acetic acid
• Soil chemical properties
• Phosphate solubilisation
• Low temperature
• Rhizobium leguminosarum subsp
• ciceri strains
• Wld chickpea
• Allochromatium vinosum
• Purple sulfur bacteria
• Anoxygenic phototrophic bacteria
• Rnf genes in Allochromatium vinosum
• Transcriptome characterization
• 454 pyrosequencing
• Gene ontology
• Molecular marker
• Transcriptome analysis
• Large scale identification
• Rhizobium leguminosarum bv
• ciceri
• Salt stress
• Wild chickpea
• Isolation and characterization
• Rhizobium associated
• Dalbergia Sissoo
• Medicago truncatula
• Nodulation specificity
• Bacterial species
• Acid production
• Biotin requirement
• Tuberose rhizoplane in raichur
• Glucose peptone broth
• Bacillus sp
• Pseudomonas sp
• Amylase production
• Identification of Bacillus species
• Potassium solubilisation