TAILIEUCHUNG - A comparison of germ tube production by Candida albicans in three media

The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liquid media were compared in ability to promote germ tube production by C. albicans. | Tr. J. of Biology 22 (1998) 205-210 © TÜBİTAK A Comparison of Germ Tube Production by Candida albicans in Three Media Gülhan VARDAR-ÜNLÜ Department of Microbiology, Faculty of Medicine, University of Cumhuriyet 58140, Sivas - TURKEY Received: Abstract: The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liquid media were compared in ability to promote germ tube production by C. albicans. Medium 199 and Glucose-Glycine Medium at 37˚C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells. Sucrose-Gelatine medium gave only 15% conversion. Glucose-Glycine medium appeared to give more rapid germ tube production than 199 Medium. Key Words: Candida albicans, germ tube, medium Üç Ortamda Candida albicans 'ın Germ Tüp Oluşumunun Kıyaslanması Özet: C. albicans' ın komersal durumdan pathojenik duruma dönüsümü, maya formundan hif (jerm tüp) formuna dimorfik geçişi yapılabilmesiyle ilgilidir. Üç sıvı ortamda Candida albicans 'ın germ tüp oluşumu uyarabilmesi için karşılaştırıldı. 199 Besiyeri ve Glukoz-Glisin Besiyeri 37˚C de oldukça başarılı sonuçlar vermiş olup, % 90 oranında maya formundan hif formuna dönüşümünü sağladı. SukrozJelatin Besiyeri sadece %15'lik dönüşüm sağladı. Glukoz-Glisin Besiyeri, 199 Besiyerin'den daha hızlı germ tüp oluşturdu. Anahtar Sözcükler: , germ tüp, besiyeri Introduction C. albicans is a opportunistic pathogen and is dimorphic. The conversion of C. albicans from the commensal to the pathogenic state is associated with transition from the yeast cell to the hyphal form (1), although the evidence in support of this is equivocal (2, 3). This phenomenon of dimorphism must involve reorganization of the cell wall at the molecular level as revealed by detection of antigens and adhesins on hyphal-form cells (4-9). The morphological development of C. albicans can be

• Sinh học
• Candida albicans
• Germ tube
• Rapid germ tube production
• Glucose Glycine medium
• Hyphal form cell
• Kỹ thuật seminested PCR
• Nấm Candida albicans
• Chủng nấm Candida albicans phân lập Việt Nam
• Phương pháp xét nghiệm chẩn đoán nấm
• Trình tự đoạn gen 410 bp của nấm Candida albicans
• Chế tạo nano berberin
• Kháng sinh thực vật
• Kháng nấm Candida albicans
• Trị bệnh do nấm Candida albicans gây ra
• Ức chế phát triển của nấm Candida albicans
• Antifungal activity
• Aspergillus fumigatus
• Essential oil
• Pathogenic fungi Candida albicans
• Essential oils from some plants
• Viêm âm đạo do nấm
• Kháng thuốc chống nấm của Candida albicans
• Kháng thuốc 5 flucytosine
• Thuốc chống nấm nhóm echinocandin
• Nghiên cứu y học
• Nhiễm nấm Candida albicans
• Định danh nấm Candida albicans
• Nhiễm trùng máu
• Sinh bài tử dày
• Tạp chí y học
• Mức độ nhạy cảm
• Candida spp với fluconazole
• Định danh các chủng candida
• Candida albicans và candida non albicans
• Immuno compromised
• Candida non albicans
• Tertiary care hospital
• Candida species amongst various clinical samples
• Native polyacrylamide gel electrophoresis
• Electrophoretic analysis
• Candida albicans Isolates
• C
• albicans strains
• Sodium dodecyl sulfate
• Germ tube production
• Retrospective study
• Hi chrome candida agar
• Various clinical samples
• Non – albicans Antifungal susceptibility
• Tertiary care teaching hospital
• Candida isolates from clinical samples
• Non albicans Candida
• Antifungal susceptibility
• Hospitalised patients
• CHROM agar
• Antifungal susceptibility testing
• Various Candida species
• Candida and its comparison
• Antifungal susceptibility profile
• Steroids or other immunosuppressive agents
• Carbohydrate polymers
• Candida spp
• Molecular weight
• Candida tropicalis
• Candida parapsilosis sensu stricto
• Diabetes mellitus
• Candida species
• Diabetes mellitus patient
• Scientific reports
• biochemistry
• molecular Biology
• oxygen species
• tumor cells
• Secreted aspartyl proteinase activity
• Acid proteinase
• Enzyme activity
• Bloodstream identified
• Kháng candida albicans
• Vi nấm nội sinh
• Nuôi cấy hoạt tính kháng
• chất kháng khuẩn
• Bảng mỏng sắc ký bằng
• Kỹ thuật hiện hình sinh học
• Genital infection
• Female rat model
• In vitro antifungal
• Biologic activity
• Immunosuppressive activity
• Bài viết về y học
• Dị hoàng thang
• Vị hoàng bá trên in vitro
• Tạo vòng kháng nấm
• Alcohol dehydrogenase
• Moonlighting proteins
• Macrophage differentiation
• ERK pathway
• Microbial growth
• Parenteral nutrition
• Blood stream infection
• Peripheral parenteral nutrition
• Pathogen isolated
• Patients attending tertiary care centre
• Candiduria cases commonly observed
• Bệnh viện Da liễu Trung ương
• Bệnh nhân viêm âm hộ
• Viêm âm đạo
• Bệnh nhiễm trùng