TAILIEUCHUNG - Electrophoretic analysis of Candida albicans Isolates collected from Turkish hospitals by Nativepolyacrylamide gel electrophoresis (N-PAGE)

Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. | Turk J Biol 24 (2000) 803–808 © TÜBİTAK Electrophoretic Analysis of Candida albicans Isolates Collected From Turkish Hospitals by NativePolyacrylamide Gel Electrophoresis (N-PAGE) Özlem OSMANAĞAOĞLU Gazi University, Faculty of Arts and Sciences, Department of Biology, Ankara-TURKEY Sefa C. SAÇILIK Ankara University, Faculty of Sciences, Department of Biology, Ankara-TURKEY Ufuk GÜNDÜZ Middle East Technical University, Faculty of Arts and Sciences, Department of Biology, Ankara-TURKEY Nurten ALTANLAR Ankara University, Faculty of Pharmacy, Department of Microbiology, Ankara-TURKEY Cumhur ÇÖKMÜŞ Ankara University, Faculty of Sciences, Department of Biology, Ankara-TURKEY Received: Abstract: Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicans strains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicans isolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. The results presented here also suggested that N-PAGE may be an efficacious method for differentiating between C. albicans strains. Türk Hastanelerinden Toplanmış Olan Candida albicans İzolatlarının Native-Poliakrilamid Jel Elektroforez ile Elektroforetik Analizi Özet: Ankara’daki hastanelerde vajinit problemli hastalardan toplanmış olan 38 adet Candida albicans suşu native-poliakrilamid jel eletroforez (N-PAGE) ile karakterize edilmiştir. C. albicans izolatlarının hücre protein ekstraktları N-PAGE’de kullanıldığı zaman üç değişik protein bant patternine rastlanmıştır. Bu çalışmanın sonucunda toplam 38 C. albicans

• Sinh học
• Native polyacrylamide gel electrophoresis
• Electrophoretic analysis
• Candida albicans Isolates
• C
• albicans strains
• Sodium dodecyl sulfate
• Occlusion body
• Lethal dose
• Lethal time
• Biological parameters
• Chromatography A
• Dispersive micro solid phase extraction
• Polydopamine coated magnetite nanoparticles
• Sodium dodecyl sulfate micellar eluents
• Antidepressant drugs
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• Tạp chí Khoa học Công nghệ và Thực phẩm
• Chất hoạt động bề mặt
• Dimidium bromide
• Linear alkylbenzene sulfonate
• Sodium lauryl ether sulfate
• Phương pháp quang phổ UV VIS
• Gram positive cocci
• Polyacrylamide gel electrophoresis
• Staphylococcus aureus
• Staphylococcus warneri
• Staphylococcus chromogenes
• Micrococcus ssp
• Coagulase negative staphylococci
• Protein profiles
• Lake Van
• Facultatively alkaliphilic Bacillus spp
• Phenotypic characterization
• Porcine pericardium
• Endothelial progenitor cells
• DNA quantification
• Mechanical properties
• Durum wheat
• Wheat landraces
• Polyphenol oxidase
• Acidic polyacrylamide gel electrophoresis
• Tạp chí Hóa học
• Cacbon hữu cơ hòa tan
• Chất hoạt động bề mặt
• Chất hoạt động natri oxalat
• Sự giải hấp thuốc trừ sâu
• Nồng độ thuốc trừ sâu
• Sự giải hấp
• Low molecular weight
• Hydroxyethyl cellulose to extract proteins in natural rubber latex
• Natural rubber latex
• The pre centrifugation latex
• Journal of Chemistry
• Carbon paste electrode
• Surface active agent
• Real sample analysis
• Antiallergic drug
• Conducting polymers
• Ion exchangers
• O aminobenzene sulfonic acid (ABS)
• Sodium dodecyl sulfate (SDS)
• Ionic liquids
• Green chemistry reagents
• Flame and electrothermal atomic absorption spectrometry
• Centaurea species
• Molecular analysis
• SDS PAGE methods
• Leuconostoc mesenteroides
• Malolactic fermentation
• Bacteriocin purification
• Diatomite calcium silicate
• Micro nano scale
• Methylene Blue
• Nano urchin like VO2
• Raman spectroscopy
• Dye contaminated water treatments
• BMC Chemistry
• Thiomolybdates solution
• Amorphous molybdenum sulfide
• Buffer solution