TAILIEUCHUNG - Availibility of use of total extracellular proteins in SDA-PAGE for characterization of gram positive cocci

There are no reports in the literature dealing with gram-positive cocci typing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study, Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus, Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). | Turk J Biol 24 (2000) 817–823 © TÜBİTAK Availibility of use of Total Extracellular Proteins in SDS-PAGE for Characterization of Gram-Positive Cocci Sefa C. SAÇILIK Ankara University, Faculty of Science, Department of Biology, Tandoğan, 06100, Ankara-TURKEY Özlem OSMANAĞAOĞLU Gazi University, Faculty of Arts and Science, Department of Biology, Teknikokullar, 06500, Ankara-TURKEY Ufuk GÜNDÜZ Middle East Technical University, Faculty of Arts and Science, Department of Biology, 06100, Ankara-TURKEY Cumhur ÇÖKMÜŞ Ankara University, Faculty of Science, Department of Biology, Tandoğan, 06100, Ankara-TURKEY Received: Abstract: There are no reports in the literature dealing with gram-positive cocci typing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study, Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus, Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). Comparison of SDSPAGE of WCP profiles with banding patterns produced by that of TEPs provided additional criteria and characteristic banding patterns for the study of the characterization of gram-positive cocci. This method was found to be easy to use and interpret and provided a rapid, reproducible and discriminative method for typing gram-positive cocci. Key Words: SDS-PAGE, gram-positive cocci, characterization. SDS-PAGE’de Hücre Dışı Proteinlerinin Gram-Pozitif Kokların Karakterizasyonu İçin Kullanılması Özet: Sodyum dodesil sülfat-poliakrilamid jel elektroforez (SDS-PAGE)’de hücre dışı proteinler (TEP) kullanılarak gram-pozitif kokların tiplendirimi ile ilgili herhangi bir rapora şu ana kadar rastlanmamıştır. Bu çalışmada; Micrococcaceae familyasına ait Staphylococcus aureus, .

• Sinh học
• Gram positive cocci
• Sodium dodecyl sulfate
• Polyacrylamide gel electrophoresis
• Staphylococcus aureus
• Staphylococcus warneri
• Staphylococcus chromogenes
• Micrococcus ssp
• Blood stream infections
• Methicillin resistant staphylococcus aureus
• Grampositive cocci
• Gram negative bacilli
• Paediatric patients admitted