TAILIEUCHUNG - Detection of prokaryotic microbial communities of Çamaltı Saltern, Turkey, by fluorescein in situ hybridization and real-time PCR
Knowledge of the functions, interactions, and diversity of extremely halophilic microorganisms mostly comes from the results of studies performed in different salterns throughout the world. As model habitats, salterns allow the comparison of different techniques used for qualitative and quantitative analysis of halophilic communities in these hypersaline environments. | M. B. MUTLU, K. GÜVEN Turk J Biol 35 (2011) 687-695 © TÜBİTAK doi: Detection of prokaryotic microbial communities of Çamaltı Saltern, Turkey, by fluorescein in situ hybridization and real-time PCR Mehmet Burçin MUTLU, Kıymet GÜVEN Biology Department, Faculty of Science, Anadolu University, Eskişehir - TURKEY Received: Abstract: Knowledge of the functions, interactions, and diversity of extremely halophilic microorganisms mostly comes from the results of studies performed in different salterns throughout the world. As model habitats, salterns allow the comparison of different techniques used for qualitative and quantitative analysis of halophilic communities in these hypersaline environments. Çamaltı Saltern is the biggest coastal solar saltern located on the Aegean coast of Turkey, and it produces most of the salt consumed in the country. In the present study, detection of prokaryotic communities of the Çamaltı Saltern was performed using 2 culture-independent methods. Real-time polymerase chain reaction (RTPCR) and fluorescein in situ hybridization (FISH) techniques were evaluated to analyze the microbial populations of Çamaltı Saltern. Of the Çamaltı samples, 48% to 67% were hybridized with the EUB338 probe and 33% to 57% were hybridized with the ARC915 probe. Repeatability of the RT-PCR experiments with environmental DNA was considered insufficient. However, FISH analysis may be combined with RT-PCR and these 2 techniques may be used in tandem to rapidly reveal quantitative aspects of the microbial population of hypersaline environments. Key words: Fluorescein in situ hybridization (FISH), real-time PCR, hypersaline environments, Çamaltı Saltern Çamaltı Tuzlası/Türkiye prokaryotik mikrobiyal kommünitelerinin fluoresan in situ hibridizasyon (FISH) ve eş zamanlı PZR teknikleri ile tespiti Özet: Ekstrem halofilik mikroorganizmaların fonksiyonları, etkileşimleri ve çeşitlilikleri üzerine bilinenler çoğunlukla dünyanın farklı .
đang nạp các trang xem trước
