TAILIEUCHUNG - Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28

The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. | Turkish Journal of Biology Turk J Biol (2013) 37: 191-198 © TÜBİTAK doi: Research Article Histology and scanning electron microscopy observations of cryopreserved protocorm-like bodies of Dendrobium sonia-28 1 2 3 1, Ranjetta POOBATHY , Uma Rani SINNIAH , Xavier RATHINAM , Sreeramanan SUBRAMANIAM * 1 School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Pulau Pinang, Malaysia 2 Department of Crop Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 3 Department of Biotechnology, AIMST University, Batu 3½, Jalan Bukit Air Nasi, 08100 Bedong, Kedah, Malaysia Received: Accepted: Published Online: Printed: Abstract: The genus Dendrobium possesses horticultural importance. Dendrobium sonia-28 is an important ornamental orchid in the flower industry. Cryopreservation is a favoured long-term storage method for orchids with propagation problems. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were cryopreserved using the vitrification technique. Histology and scanning electron microscopy (SEM) observations were conducted on stock, non-cryopreserved (control), and cryopreserved PLBs of Dendrobium sonia-28 to detect cryoinjuries resulting from the vitrification protocol. Histological observations of control PLBs indicated that the preculture, osmoprotection, and dehydration steps were not physically damaging to the PLBs. Histological and SEM analyses of cryopreserved PLBs indicated that the freezing and thawing cycles inflicted damages on the parenchymatic regions of the PLBs. Only embryogenic cells survived the treatment. Scanning electron microscopy studies of the control and cryopreserved PLBs indicated that both osmotic and freezing injuries occurred only in the interior regions of the PLBs. Key words: Cryopreservation, Dendrobium sonia-28, histology, protocorm-like bodies, scanning electron microscopy, .

• Sinh học
• Dendrobium sonia 28
• Protocorm like bodies
• Scanning electron microscopy
• Liquid nitrogen
• Tertiary butyl alcohol