TAILIEUCHUNG - Ebook PCR Troubleshooting and Optimization

The polymerase chain reaction (PCR) has become a íundamental tool in molecular research and clinical testing. The origins of PCR and its early evolution are described; including adaptation to RNA; thermostable polymerases, automation, improvements in speciíkity and rapid temperature cycling. | PCR Troubleshooting and Optimization The Essential Guide Caister Academic Press k Copyright 201 1 Caister Academic Press Norfolk UK British Library Cataloguing-in-Publication Data A catalogue record for tlìis book is available from the British Library ISBN 978-1-904455-72-1 Description or mention of instrumentation soítvvare or other Products in this book does not imply endorsement by the author or publisher. The author and publisher do not assume responsibility for the validity of any products or procedures mentioned or described in this book or for the consequences of their use. All rights reserved. No part of this publication may be reproduced stored in a retrieval System or transmitted in any íorm or by any means electronic mechanical photocopying recording or othervvise vvithout the prior permission of the publisher. No cỉaim to original . Government works. Cover image adapted hom Pigure Printed and bound in Great Britain Contents List of Contributors V 1 2 3 4 5 6 7 8 9 10 11 Magic in Solution an Introduction and Brief History of PCR 1 Carl T. VVittvver and Jared s. Farrar qPCR Inhibition and Amplification of Difficult Templates 23 Jack M. Gailup Signiticance of Controls and Standard Curves in PCR 67 lan Kavanagh Gervvyn Jones and Saima N. Nayab Obtaining Maximum PCR Sensitivity and Speciticity 79 Cameron N. Gundry and Matthevv D. Poulson RT-PCR Optimization Strategies 97 Real-Time PCR Instrumentation an Instrument Selection Guide 119 Sandrine Javorschi-Miller and Ivan Delgado Orlic Quantitative PCR Data Analysis - Unlockinq the Secret to Successtul Results 139 Jan Hellemans and Jo Vandesompeỉe The MIQE Guidelines Uncloaked 151 Greg Shiplev PCR Applications for Epigenetics Research 167 Gavin Meredith Miroslav Dudas Mark Landers Vasiliki Anest Jonathan Wang Caitu Chen Peter Jozsi and Christopher Adams High-Resolution Melting Analysis 201 John F. Mackay and Carl T. Wittwer Microtluidic Emulsion PCR 217 N. Reginald Beer and John H. Leamon

• Sinh học
• PCR Troubleshooting and Optimization
• The polymerase chain reaction
• The thermostable polymerases
• Rapid temperature cycling
• Công nghệ polymer
• Ebook PCR Troubleshooting and Optimization