TAILIEUCHUNG - Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.)

Department of Biochemistry, University of Rajshahi, Rajshahi-6205. Bangladesh Three lectins were extracted and purified from mulberry seeds by gel filtration of 100% ammonium sulfate saturated crude protein extract followed by ion-exchange chromatography on DEAE and CM-cellulose. The lectins were found to be homogeneous as judged by polyacrylamide disc gel electrophoresis. The molecular masses of the lectins as determined by gel filtration were 175 000 for MSL-1, 120 000 for MSL-2 and 89 500 for MSL-3 | Eur. J. Biochem. 268 6005-6010 2001 FEBS 2001 Purification and characterization of three galactose specific lectins from Mulberry seeds Morus sp. Tanzima Yeasmin Md Abul Kashem Tang Abdur Razzaque and Nurul Absar Department of Biochemistry University of Rajshahi Rajshahi-6205. Bangladesh Three lectins were extracted and purified from mulberry seeds by gel filtration of 100 ammonium sulfate saturated crude protein extract followed by ion-exchange chromatography on DEAE and CM-cellulose. The lectins were found to be homogeneous as judged by polyacrylamide disc gel electrophoresis. The molecular masses of the lectins as determined by gel filtration were 175 000 for MSL-1 120 000 for MSL-2 and 89 500 for MSL-3. MSL-1 is dimer in nature with the two monomers held together by disulfide bond s while MSL-2 and MSL-3 contain four nonidentical subunits that are held together by nonionic hydrophobic interactions. The lectins agglutinated rat red blood cells and this agglutination was inhibited specifically by galactose methyl-a-D-galactopyranoside methyl-p-D-galactopyrano-side lactose and raffinose. The lectins MSL-1 MSL-2 and MSL-3 contained and neutral sugars respectively and the sugar composition of the lectins was glucose and mannose for MSL-1 and galactose for both MSL-2 and MSL-3. The lectins exhibited strong cytotoxic effect in brine shrimp lethality bioassay. Keywords mulberry seeds galactose-specific lectins subunit structure hemagglutination cytotoxicity. Mulberry Morus alba L. is the sole host plant of the silkworm Bombyx mori Linn which produces silk. It belongs to the family Moraceae part of the genus Morus. It is a deep rooted perennial plant widely distributed in Asia Europe Africa and Latin America in a wide range of climatic conditions varying from temperate to tropical. The silkworm can only obtain nutrients necessary for growth from mulberry leaves. Lectin isolated chiefly from plants bacteria fungi invertebrates and vertebrates are .

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