TAILIEUCHUNG - Báo cáo Y học: The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus

We have recently expressed in bacteria the enzymatically active reverse transcriptase (RT) of bovine leukemia virus (BLV) [Perach, M. & Hizi, A. (1999) Virology 259, 176–189]. In the present study, we have studied in vitro two features of the DNA polymerase activity of BLV RT, the processivity of DNA synthesis and the fidelity of DNA synthesis. These properties were compared with those of the well-studied RTs of human immunodeficiency virus type 1 (HIV-1) and murine leukaemia virus (MLV). | Eur. J. Biochem. 269 859-867 2002 FEBS 2002 The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus Orna Avidan Michal Entin Meer Iris Oz and Amnon Hizi Department of Cell Biology and Histology Sackler School of Medicine Tel Aviv University Tel Aviv Israel We have recently expressed in bacteria the enzymatically active reverse transcriptase RT of bovine leukemia virus BLV Perach M. Hizi A. 1999 Virology 259 176-189 . In the present study we have studied in vitro two features of the DNA polymerase activity of BLV RT the processivity of DNA synthesis and the fidelity of DNA synthesis. These properties were compared with those of the well-studied RTs of human immunodeficiency virus type 1 HIV-1 and murine leukaemia virus MLV . Both the elongation of the DNA template and the processivity of DNA synthesis exhibited by BLV RT are impaired relative to the other two RTs studied. Two parameters of fidelity were studied the capacity to incorporate incorrect nucleotides at the 3 end of the nascent DNA strand and the ability to extend these 3 end mispairs. BLV RT shows a fidelity of misinsertion higher than that of HIV-1 RT and lower than that of MLV RT. The pattern of mispair elongation by BLV RT suggests that the in vitro error proneness of BLV RT is closer to that of HIV-1 RT. These fidelity properties are discussed in the context of the various retroviral RTs studied so far. Keywords bovine leukaemia virus fidelity processivity reverse transcriptase. Bovine leukaemia virus BLV is a naturally occurring exogenous B-cell lymphotropic retrovirus which is the aetiological agent of cattle leukosis. This disease is characterized by an initial persistent lymphocytosis which is followed by the occurrence of clonal lymphoid B-cell tumours after a long latency period 1 . This virus is related to human T-cell leukemia viruses type I and type II HTLV-I and HTLV-II respectively forming a subfamily of transactivating retroviruses 2 .

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