TAILIEUCHUNG - Báo cáo Y học: The function of methyl-menaquinone-6 and polysul®de reductase membrane anchor (PsrC) in polysul®de respiration ofWolinella succinogenes

Wolinella succinogenesgrows by oxidative phosphorylation with polysul®de as terminal electron acceptor and either H2 or formate as electron donor (polysul®de respiration). The function of the respiratory chains catalyzing these reactions was investigated. Proteoliposomes containing polysul®de reductase (Psr) and either hydrogenase or formate dehy-drogenase isolated fromthemembrane fraction ofWolinella succinogenescatalyzed polysul®de respiration, provided that methyl-menaquinone-6 isolated fromW. . | Eur. J. Biochem. 269 1086-1095 2002 FEBS 2002 The function of methyl-menaquinone-6 and polysulfide reductase membrane anchor PsrC in polysulf de respiration of Wolinella succinogenes Wiebke Dietrich and Oliver Klimmek Institut fur Mikrobiologie Johann Wolfgang Goethe-Universitat Frankfurt am Main Germany Wolinella succinogenes grows by oxidative phosphorylation with polysulfde as terminal electron acceptor and either H2 or formate as electron donor polysulfde respiration . The function of the respiratory chains catalyzing these reactions was investigated. Proteoliposomes containing polysulfde reductase Psr and either hydrogenase or formate dehydrogenase isolated from the membrane fraction of Wolinella succinogenes catalyzed polysulfide respiration provided that methyl-menaquinone-6 isolated from W. succinogenes was also present. The specific activities of electron transport were commensurate with those of the bacterial membrane fraction. Using site-directed mutagenesis certain residues were substituted in PsrC the membrane anchor of polysulfide reductase. Replacement of Y23 D76 Y159 D218 E225 or R305 caused nearly full inhibition of polysulfide respiration without affecting the activity of Psr which was still bound to the membrane. These residues are predicted to be located in hydrophobic helices of PsrC or next to them. Substitution of 13 other residues of PsrC either caused partial inhibition of polysulfide respiration or had no effect. The function of methyl-menaquinone-6 which is thought to be bound to PsrC is discussed. Keywords methyl-menaquinone polysulfde respiration sulfur respiration hydrogenase formate dehydrogenase. Wolinella succinogenes grows at the expense of polysulfide S respiration with H2 reaction a or formate reaction b as electron donor 1-3 . Reactions a AGo -31 kJ-mol H2 1 and b AGo - 30 kJ mol-1 formate are coupled to the generation of an electrochemical proton potential Ap V across the bacterial membrane which drives ATP synthesis 3-6 .

• Báo cáo khoa học
• Traditional medicine
• Report medicine
• scientific reports
• Crystal structure
• medical analysis
• biochemical studies
• Commercial use
• Tadehagi triquetrum (L ) H Ohashi
• Indigenous medicine in Myanmar
• Medicinal plants
• Calotropis gigantea R
• Br
• Traditional medicinal plants
• Local development in Myanmar
• Important role in health care system
• BMC Chemistry
• Trace analysis
• Dispersive solvent
• Traditional Chinese medicine
• Chinese medicine oral liquid
• Veterinary Medicine
• Herbal Medicine
• Varughese George
• Andrographis paniculata
• Chemical Constituents
• alternative medicine
• diagnostic techniques
• Diagnostic therapies
• Chinese medicine
• Medical herbalism
• Massage therapy review
• Lecture Massage therapy review
• Bodywork assessment
• Massage therapy
• Traditional chinese medicine modality
• Acanthospermum hispidum
• Burkina faso
• Pharmacognosic characterization
• Acanthospermum hispidum (Asteraceae)
• Medicinal plant widely
• Active components
• Plasma protein binding
• Research methods
• Doctoral thesis abstract
• Improving accounting work organization
• Accounting work organization
• Traditional medicine hospitals
• Hanoi city
• Vietnamese traditional medicine
• Medicinal herbs
• Ethnic groups
• Community healthcare
• Mountainous area
• Cultural Perspective
• Modern Medicine
• Funding Perspective
• Traditional Oriental
• medicine
• cell proliferation
• oxidation
• breast cancer
• chemical reaction
• gastric cancer
• hormone medicine
• tumor cells
• environmental medicine