TAILIEUCHUNG - Báo cáo khoa học: P NMR studies of energy metabolism in xanthosine5¢-monophosphate overproducing Corynebacterium ammoniagenes

Corynebacterium ammoniagenesis an overproducer of xanthosine-5¢-monophosphate (XMP) by consuming either glcose (glc) or glutamic acid (glu). Its energymetabolismwas studiedin vivousing 31 P NMR spectroscopy coupled with a circulating fermentation system (CFS). CFS enabled us to validate directly the cellular dependency on carbon sources and changes in biomolecules produced according to altera-tions in the cellular energetic status. | Eur. J. Biochem. 270 2622-2626 2003 FEBS 2003 doi 31P NMR studies of energy metabolism in xanthosine-5 -monophosphate overproducing Corynebacterium ammoniagenes Yasushi Noguchi1 Nobuhisa Shimba2 Yoshio Kawahara1 Ei-ichiro Suzuki2 and Shinichi Sugimoto1 1 Fermentation Biotechnology Laboratories and 2Central Research Laboratories Ajinomoto Co. Inc. Kawasaki-ku Kawasaki Kanagawa Japan Corynebacterium ammoniagenes is an overproducer of xanthosine-5 -monophosphate XMP by consuming either glcose glc or glutamic acid glu . Its energy metabolism was studied in vivo using 31P NMR spectroscopy coupled with a circulating fermentation system CFS . CFS enabled us to validate directly the cellular dependency on carbon sources and changes in biomolecules produced according to alterations in the cellular energetic status. For the most efficient XMP production the glutamic acid and glcose molar ratios glu glc in the medium were adjusted to a molar ratio of . The 31P NMR illustrated the two distinct phases of the cellular energetic status due to the availability of the substrates from the medium. In the earlier phase both glc and glu were utilized resulting in average ATP and ADP concentrations in cells of Ltmol-g 1 of dry cell weight DCW and an undetermined level respectively. The ADP concentration in the later phase increased to Ltmol-g 1 of DCW while the ATP concentration decreased to an undetectable level in association with a remarkable decrease in XMP production. This decrease in the XMP-producing ability was associated with an increase in production of the by-product hypoxanthine. Because glu was found to be consumed completely during the earlier phase glc was the only available substrate in the later phases. These findings by in vivo NMR indicate that changes in the carbon metabolism profoundly affect XMP production by C. ammoniagenes. Keywords xanthosine-5 -monophosphate in vivo NMR energy metabolism Corynebacterium .

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