TAILIEUCHUNG - Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region

The peptides of the neuropeptide Y (NPY) family exert their functions, including regulation of appetite and circadian rhythm, by binding to G-protein coupled receptors. Mammals have five subtypes, named Y1,Y2 , Y4,Y5 and Y6, and recently Y7 has been discovered in fish and amphibi-ans. In chicken we have previously characterized the first four subtypes and here we describe Y6 and Y7. | iFEBS Journal Neuropeptide Y-family receptors Y6 and Y7 in chicken Cloning pharmacological characterization tissue distribution and conserved synteny with human chromosome region Torun Bromíée 1 Paula Sjõdin q Robert Fredriksson1 Tim Boswell2 Tomas A. Larsson1 Erik Salaneck1 Rima Zoorob3 Nina Mohell1 and Dan Larhammar1 1 Department of Neuroscience Unit of Pharmacology Uppsala University Sweden 2 Roslin Institute Edinburgh Roslin UK 3 Institut Andre Lwoff Unite de Genetique Moleculaire et Integration des Fonctions Cellulaire Villejuif France Keywords G-protein coupled receptor NPY paralogon PYY synteny Correspondence Dan Larhammar Department. of Neuroscience Unit of Pharmacology Uppsala University Box 593 SE-75124 Uppsala Sweden Fax 46 18 511540 Tel 46 18 4714173 E-mail Website http danl The authors contributed equally to this paper Received 8 September 2005 revised 24 February 2006 accepted 9 March 2006 doi The peptides of the neuropeptide Y NPY family exert their functions including regulation of appetite and circadian rhythm by binding to G-protein coupled receptors. Mammals have five subtypes named Y1 Y2 Y4 Y5 and Y6 and recently Y7 has been discovered in fish and amphibians. In chicken we have previously characterized the first four subtypes and here we describe Y6 and Y7. The genes for Y6 and Y7 are located 1 megabase apart on chromosome 13 which displays conserved synteny with human chromosome 5 that harbours the Y6 gene. The porcine PYY radioligand bound the chicken Y6 receptor with a Kd of nM. No functional coupling was demonstrated. The Y6 mRNA is expressed in hypothalamus gastrointestinal tract and adipose tissue. Porcine PYY bound chicken Y7 with a Kd of nM mean SEM whereas chicken PYY surprisingly had a much lower affinity with a Ki of 41 nM perhaps as a result of its additional amino acid at the N terminus. Truncated peptide fragments had greatly reduced .

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