TAILIEUCHUNG - Báo cáo khoa học: Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family

Among the RFamide peptide groups, PQRFamide peptides, such as neuro-peptide FF (NPFF) and neuropeptide AF (NPAF), share a common C-ter-minal Pro-Gln-Arg-Phe-NH2 motif. LPXRFamide (X¼L or Q) peptides, such as gonadotropin-inhibitory hormone (GnIH), frog growth hormone-releasing peptide (fGRP), goldfish LPXRFamide peptide and mammalian RFamide-related peptides (RFRPs), also share a C-terminal Leu-Pro-Leu⁄Gln-Arg-Phe-NH2 motif. | ềFEBS Journal Evolutionary origin and divergence of PQRFamide peptides and LPXRFamide peptides in the RFamide peptide family Insights from novel lamprey RFamide peptides Tomohiro Osugi1 Kazuyoshi Ukena1 Stacia A. Sower2 Hiroshi Kawauchi3 and Kazuyoshi Tsutsui1 1 Laboratory of Brain Science Faculty of Integrated Arts and Sciences Hiroshima University Japan 2 Department of Biochemistry and Molecular Biology University of New Hampshire Durham USA 3 Laboratory of Molecular Endocrinology Schoolof Fisheries Sciences Kitasato University Iwate Japan Keywords molecular evolution agnathan LPXRFamide peptide PQRFamide peptide neuropeptide FF Correspondence K. Tsutsui Laboratory of Brain Science Faculty of Integrated Arts and Sciences Hiroshima University Higashi-Hiroshima 739-8521 Japan Fax 81 82 424 0759 Tel 81 82 424 6571 E-mail tsutsui@ Database The nucleotide sequence data of lamprey PQRFa has been submitted to the DDBJ EMBL and GenBank Nucleotide Sequence Databases under Accession no. AB233469. Received 12 December 2005 revised 14 February 2006 accepted 20 February 2006 doi Among the RFamide peptide groups PQRFamide peptides such as neuropeptide FF NPFF and neuropeptide AF NPAF share a common C-ter-minal Pro-Gln-Arg-Phe-NH2 motif. LPXRFamide X L or Q peptides such as gonadotropin-inhibitory hormone GnIH frog growth hormone-releasing peptide fGRP goldfish LPXRFamide peptide and mammalian RFamide-related peptides RFRPs also share a C-terminal Leu-Pro-Leu Gln-Arg-Phe-NH2 motif. Such a similar C-terminal structure suggests that these two groups may have diverged from a common ancestral gene. In this study we sought to clarify the evolutionary origin and divergence of these two groups by identifying novel RFamide peptides from the brain of sea lamprey one of only two extant groups of the oldest lineage of vertebrates Agnatha. A novel lamprey RFamide peptide was identified by immunoaffinity purification using the antiserum .

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