TAILIEUCHUNG - Báo cáo khoa học: Phytochelatin–cadmium–sulfide high-molecular-mass complexes of Euglena gracilis

High-molecular-mass PC complexes (PC-HMWCs) constituted by phyto-chelatins (PCs), cadmium and sulfide are synthesized by several organisms after exposure to cadmium. In this study, PC-HMWCs were isolated from photoheterotrophicEuglena gracilisand purified to homogeneity, resulting in compounds of molecular mass 50–380 kDa depending on the CdCl2and sulfate concentrations in the culture medium. | ỊFEBS Journal Phytochelatin-cadmium-sulfide high-molecular-mass complexes of Euglena gracilis David G. Mendoza-Cozatl1 Jose S. Rodriguez-Zavala1 Sara Rodriguez-Enriquez1 Guillermo Mendoza-Hernandez2 Roberto Briones-Gallardo3 and Rafael Moreno-Sanchez1 1 Institute Nacionalde Cardiologia Departamento de Bioquimica Mexico . Mexico 2 Universidad NacionalAutonoma de Mexico Departamento de Bioquimica Facultad de Medicina Mexico . Mexico 3 Universidad Autonoma de San Luis Potosí Facultad de Ingenieria-Instituto de Metalurgia San Luis Potosí Mexico Keywords aspartate cadmium compartmentation chloroplast glutathione sulfide Correspondence R. Moreno-Sanchez Departamento de Bioquimica Instituto Nacionalde Cardiologia Juan Badiano 1 Col. Seccion XVI Tlalpan 14080 Mexico . Mexico Fax 52 55 5573 0926 Tel 52 55 5573 2911 Ext. 1422 E-mail morenosanchez@ Received 1 September 2006 revised 23 October 2006 accepted 26 October 2006 doi High-molecular-mass PC complexes PC-HMWCs constituted by phyto-chelatins PCs cadmium and sulfide are synthesized by several organisms after exposure to cadmium. In this study PC-HMWCs were isolated from photoheterotrophic Euglena gracilis and purified to homogeneity resulting in compounds of molecular mass 50-380 kDa depending on the CdCl2 and sulfate concentrations in the culture medium. In contrast with plants and some yeasts PC-HMWCs from E. gracilis mainly comprise 57-75 monothiol molecules Cys y-glutamylcysteine GSH and to a lesser extent 25-43 PCs. A similar acid-soluble thiol compound composition was found in whole cell extracts. The -SH Cd2 and S2 Cd2 ratios found in purified PC-HMWCs were and respectively the -SH S2- Cd2 ratio was . PC-HMWCs of molecular mass 60 and 100 kDa were also localized inside Percoll-purified chloroplasts in which cadmium and PCs were mainly compartmentalized. Cadmium and sulfur-rich clusters with similar sulfur cadmium stoichiometries to those of the .

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