TAILIEUCHUNG - Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa

Odorant binding proteins (OBP) are soluble lipocalins produced in large amounts in the nasal mucosa of several mammalian species. Although OBPs can bind a large variety of odorous compounds, direct and exclusive involvement of these proteins in olfactory perception has not been clearly demonstrated. | ỊFEBS Journal Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa Stefano Grolli Elisa Merli Virna Conti Erika Scaltriti and Roberto Ramoni Dipartimento di Produzioni Animali Biotecnologie Veterinarie Qualita e Sicurezza degli Alimenti Universita degli Studi di Parma Italy Keywords 4-hydroxy-2-nonenal lipocalins odorant binding protein oxidative stress reactive oxygen species Correspondence R. Ramoni Dipartimento di Produzioni Animali Biotecnologie Veterinarie Qualita e Sicurezza degli Alimenti University di Parma Via delTaglio 8 43100 Parma Italy Fax 39 052 190 2770 Tel 39 052 190 2767 E-mail vetbioc@ Received 7 June 2006 revised 18 August 2006 accepted 21 September 2006 doi Odorant binding proteins OBP are soluble lipocalins produced in large amounts in the nasal mucosa of several mammalian species. Although OBPs can bind a large variety of odorous compounds direct and exclusive involvement of these proteins in olfactory perception has not been clearly demonstrated. This study investigated the binding properties and chemical resistance of OBP to the chemically reactive lipid peroxidation end-product 4-hydroxy-2-nonenal HNE in an attempt to establish a functional relationship between this protein and the molecular mechanisms combating free radical cellular damage. Experiments were carried out on recombinant porcine and bovine OBPs and results showed that both forms were able to bind HNE with affinities comparable with those of typical OBP ligands Kd and pM for porcine and bovine OBP respectively . Furthermore OBP functionality as determined by measuring the binding of the fluorescent ligand 1-aminoanthracene was partially lost only when incubating HNE levels and exposure time to HNE exceeded physiological values in nasal mucosa. Finally preliminary experiments in a simplified model resembling nasal epithelium showed that extracellular OBP can .

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