TAILIEUCHUNG - Báo cáo khoa học: Roles of 1-Cys peroxiredoxin in haem detoxification in the human malaria parasite Plasmodium falciparum

In the present study, we investigated whetherPlasmodium falciparum1-Cys peroxiredoxin (Prx) (Pf1-Cys-Prx), a cytosolic protein expressed at high lev-els during the haem-digesting stage, can act as an antioxidant to cope with the oxidative burden of haem (ferriprotoporphyrin IX; FP). Recombinant Pf1-Cys-Prx protein (rPf1-Cys-Prx) competed with glutathione (GSH) for FP and inhibited FP degradation by GSH. When rPf1-Cys-Prx was added to GSH-mediated FP degradation, the amount of iron released was reduced to 23% of the reaction without the protein (P. | iFEBS Journal Roles of 1-Cys peroxiredoxin in haem detoxification in the human malaria parasite Plasmodium falciparum Shin-ichiro Kawazu1 2 Nozomu Ikenoue1 Hitoshi Takemae1 2 Kanako Komaki-Yasuda1 2 and Shigeyuki Kano1 1 Research Institute InternationalMedicalCenter of Japan Tokyo Japan 2 Precursory Research for Embryonic Science and Technology Japan Science and Technology Agency Saitama Japan Keywords glutathione haem malaria peroxiredoxin Plasmodium falciparum Correspondence . Kawazu Research Institute International Medical Center of Japan 1-21-1 Toyama Shinjuku-ku Tokyo 162-8655 Japan Fax 81 3 3202 7364 Tel 81 3 3202 7181 extn 2878 E-mail skawazu@ Received 30 December 2004 revised 9 February 2005 accepted 14 February 2005 doi In the present study we investigated whether Plasmodium falciparum 1-Cys peroxiredoxin Prx Pfl-Cys-Prx a cytosolic protein expressed at high levels during the haem-digesting stage can act as an antioxidant to cope with the oxidative burden of haem ferriprotoporphyrin IX FP . Recombinant Pf1-Cys-Prx protein rPf1-Cys-Prx competed with glutathione GSH for FP and inhibited FP degradation by GSH. When rPf1-Cys-Prx was added to GSH-mediated FP degradation the amount of iron released was reduced to 23 of the reaction without the protein P . The rPfl-Cys-Prx bound to FP-agarose at pH which is the pH of the parasite cytosol. The rPf1-Cys-Prx could completely protect glutamine synthetase from inactivation by the dithiothreitol-Fe3 -dependent mixed-function oxidation system and it also protected enolase from inactivation by coincubation with FP GSH. Incubation of white ghosts of human red blood cells and FP with rPf1-Cys-Prx reduced formation of membrane associations with FP to 75 of the incubation without the protein P . The findings of the present study suggest that Pf1-Cys-Prx protects the parasite against oxidative stresses by binding to FP slowing the rate of GSH-medi-ated FP .

TÀI LIỆU LIÊN QUAN
TÀI LIỆU MỚI ĐĂNG
11    163    2    23-12-2024
TAILIEUCHUNG - Chia sẻ tài liệu không giới hạn
Địa chỉ : 444 Hoang Hoa Tham, Hanoi, Viet Nam
Website : tailieuchung.com
Email : tailieuchung20@gmail.com
Tailieuchung.com là thư viện tài liệu trực tuyến, nơi chia sẽ trao đổi hàng triệu tài liệu như luận văn đồ án, sách, giáo trình, đề thi.
Chúng tôi không chịu trách nhiệm liên quan đến các vấn đề bản quyền nội dung tài liệu được thành viên tự nguyện đăng tải lên, nếu phát hiện thấy tài liệu xấu hoặc tài liệu có bản quyền xin hãy email cho chúng tôi.
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.