TAILIEUCHUNG - Báo cáo khoa học: Reconstruction ofde novopathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose inSaccharomyces cerevisiae

UDP-d-glucuronic acid and UDP-d-xylose are required for the biosynthesis of glycosaminoglycan in mammals and of cell wall polysaccharides in plants. Given the importance of these glycans to some organisms, the development of a system for production of UDP-d-glucuronic acid and UDP-d-xylose from a common precursor could prove useful for a number of applications. | ềFEBS Journal Reconstruction of de novo pathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose in Saccharomyces cerevisiae Takuji Oka and Yoshifumi Jigami Research Center for Glycoscience National institute of Advanced Industrial science and Technology AIST Tsukuba Japan Keywords Saccharomyces cerevisiae UDP-glucuronic acid UDP-glucuronic acid decarboxylase UDP-glucose dehydrogenase UDP-xylose Correspondence Y. Jigami Research Center for Glycoscience National institute of Advanced Industrial science and Technology AIST AISTTsukuba Central6 Higashi 1-1 Tsukuba 305-8566 Japan Fax 81 29 861 6161 Tel 81 29 861 6160 E-mail Received 27 February 2006 revised 6 April 2006 accepted 12 April 2006 doi UDP-D-glucuronic acid and UDP-D-xylose are required for the biosynthesis of glycosaminoglycan in mammals and of cell wall polysaccharides in plants. Given the importance of these glycans to some organisms the development of a system for production of UDP-D-glucuronic acid and UDP-D-xylose from a common precursor could prove useful for a number of applications. The budding yeast Saccharomyces cerevisiae lacks an endogenous ability to synthesize or consume UDP-D-glucuronic acid and UDP-D-xylose. However yeast have a large cytoplasmic pool of UDP-d-glucose that could be used to synthesize cell wall b-glucan as a precursor of UDP-D-glucuronic acid and UDP-D-xylose. Thus if a mechanism for converting the precursors into the end-products can be identified yeast may be harnessed as a system for production of glycans. Here we report a novel S. cerevisiae strain that coexpresses the Arabidopsis thaliana genes UGD1 and UXS3 which encode a UDP-glucose dehydrogenase AtUGD1 and a UDP-glucuronic acid decarboxylase AtUXS3 respectively which are required for the conversion of UDP-D-glucose to UDP-D-xylose in plants. The recombinant yeast strain was capable of converting UDP-D-glucose to UDP-D-glucuronic .

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