TAILIEUCHUNG - Báo cáo khoa học: EcR expression in the prothoracicotropic hormoneproducing neurosecretory cells of the Bombyx mori brain

The steroid hormone 20-hydroxyecdysone (20E) initiates insect molting and metamorphosis through binding with a heterodimer of two nuclear recep-tors, the ecdysone receptor (EcR) and ultraspiracle (USP). Expression of the specific isoforms EcR-A and EcR-B1 governs steroid-induced responses in the developing cells of the silkworm Bombyx mori. | ỊFEBS Journal EcR expression in the prothoracicotropic hormone-producing neurosecretory cells of the Bombyx mori brain An indication of the master cells of insect metamorphosis Monwar Hossain1 Sakiko Shimizu2 Haruhiko Fujiwara3 Sho Sakurai1 2 and Masafumi Iwami1 2 1 Division of Life Sciences Graduate Schoolof NaturalScience and Technology Kanazawa University Japan 2 Division of BiologicalScience Graduate Schoolof NaturalScience and Technology Kanazawa University Japan 3 Department of Integrated Biosciences Graduate Schoolof Frontier Sciences The University of Tokyo Kashiwa Japan Keywords Bombyx mori brain ecdysone receptor metamorphosis prothoracicotropic hormone-producing neurosecretory cell Correspondence M. Iwami Division of Life Sciences Graduate School of Natural Science and Technology Kanazawa University Kanazawa 920-1192 Japan Fax 81 76 2646251 Tel 81 76 2646255 E-mail masafumi@ Received 18 May 2006 revised 22 June 2006 accepted 26 June 2006 doi The steroid hormone 20-hydroxyecdysone 20E initiates insect molting and metamorphosis through binding with a heterodimer of two nuclear receptors the ecdysone receptor EcR and ultraspiracle USP . Expression of the specific isoforms EcR-A and EcR-B1 governs steroid-induced responses in the developing cells of the silkworm Bombyx mori. Here analysis of EcR-A and EcR-B1 expression during larval-pupal development showed that both genes were up-regulated by 20E in the B. mori brain. Wholemount in situ hybridization and immunohistochemistry revealed that EcR-A and EcR-B1 mRNAs and proteins were exclusively located in two pairs of lateral neurosecretory cells in the larval brain known as the pro-thoracicotropic hormone PTTH - producing cells PTPCs . In the pupal brain EcR-A and EcR-B1 expression was detected in tritocerebral cells and optic lobe cells in addition to PTPCs. As PTTH controls ecdysone secretion by the prothoracic gland these results indicate that .

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