TAILIEUCHUNG - Báo cáo khoa học: Recent applications of NMR spectroscopy in plant metabolomics

Recent research has established NMR as a key method for high-through-put comparative analysis of plant extracts. We discuss recent examples of the use of NMR to provide metabolomic data for various applications in plant science and look forward to the key role that NMR will play in data provision for plant systems biology. | MINIREVIEW Recent applications of NMR spectroscopy in plant metabolomics Jane L. Ward John M. Baker and Michael H. Beale The National centre for Plant and MicrobialMetabolomics Rothamsted Research Harpenden UK Keywords fingerprinting NMR plant metabolomics substantial equivalence Recent research has established NMR as a key method for high-throughput comparative analysis of plant extracts. We discuss recent examples of the use of NMR to provide metabolomic data for various applications in Correspondence M. Beale The National centre for Plant and Microbial Metabolomics Rothamsted Research West Common Harpenden AL5 2JQ UK Tel 44 1582 763133 E-mail plant science and look forward to the key role that NMR will play in data provision for plant systems biology. Received 19 October 2006 revised 15 November 2006 accepted 20 November 2006 doi Introduction Nuclear magnetic resonance NMR spectroscopy is usually the method of choice for natural product structure determination and it is not surprising that this powerful technique has come to the fore in plant meta-bolomics. The data requirements for metabolomics are the qualitative and quantitative analyses of the maximum number of metabolites in the highest achievable throughput. Most metabolomics laboratories deploy a range of spectroscopic technologies but use of NMR spectroscopy particularly as a first pass screen has a number of advantages over other analytical platforms currently being used. Sample preparation is relatively simple when compared to other analytical methods and a high sample throughput with little instrument drift is readily achieved. NMR is not discriminatory unlike certain mass spectrometry methods that rely on the prior derivatization of metabolites or the ability of them to ionize. Metabolite screening requires maximum sensitivity with a broad compound coverage. For NMR this usually means that only the most sensitive and commonly occurring magnetic

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