TAILIEUCHUNG - Báo cáo khoa học: Sherlock Holmes and the proteome ) a detective story Pier Giorgio Righetti1 and Egisto Boschetti2

The performance of a hexapeptide ligand library in capturing the ‘hidden proteome’ is illustrated and evaluated. This library, insolubilized on an organic polymer and available under the trade name ‘Equalizer Bead Tech-nology’, acts by capturing all components of a given proteome, by concen-trating rare and very rare proteins, and simultaneously diluting the abundant ones. | ỊFEBS Journal REVIEW ARTICLE Sherlock Holmes and the proteome - a detective story Pier Giorgio Righetti1 and Egisto Boschetti2 1 Department of Chemistry Materials and ChemicalEngineering Giulio Natta Polytechnic of Milano Milan Italy 2 Ciphergen Biosystems Fremont CA USA Keywords E. coli proteome ligand library peptide ligands rare proteome S. cerevisiae proteome urine and serum analysis Correspondence P. G. Righetti Department of Chemistry Materials and ChemicalEngineering Giulio Natta Polytechnic of Milano Via Mancinelli 7 Milano 20133 Italy Fax 39 022399 3080 Tel 39 022399 3016 E-mail Note This lecture was delivered at the 7th Siena Meeting From Genome to Proteome Back to the Future September 3-7 2006 Siena Italy. Received 5 October revised 26 November 2006 accepted 13 December 2006 The performance of a hexapeptide ligand library in capturing the hidden proteome is illustrated and evaluated. This library insolubilized on an organic polymer and available under the trade name Equalizer Bead Technology acts by capturing all components of a given proteome by concentrating rare and very rare proteins and simultaneously diluting the abundant ones. This results in a proteome of normalized relative abundances amenable to analysis by MS and any other analytical tool. Examples are given of analysis of human urine and serum as well as cell and tissue lysates such as Escherichia coli and Saccharomyces cerevisiae extracts. Another important application is impurity tracking and polishing of recombinant DNA products especially biopharmaceuticals meant for human consumption. doi Introduction The word detective originates from the Latin detego detexi detectum detegere . to find out to discover in fact to remove the teges or tegmen in English slang the cover therefore to uncover . Modern prote-ome analysis is a very complex detective story which might baffle even the most famous investigator Sherlock Holmes 1 . .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• biological activities
• gastric cancer
• medical knowledge
• medical research
• analysis of cytoplasmic
• oxidation
• Crystal structure
• bacteria
• hemoglobin
• medicine
• cell proliferation
• chemical reaction
• hormone medicine
• tumor cells
• biochemical studies
• environmental medicine