TAILIEUCHUNG - Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB

In an attempt to isolate lipopolysaccharide fromSpirocha-eta aurantia, Darveau-Hancock extraction of the cell mass was lipopolysaccharidewas found, two carbohydrate-containing compounds were detected. They were resolved by size-exclusion chromatography into high molecularmass(LGLA) and low molecular mass (LGLB) fractions. Here we present the results of the analysis of the glycolipid LGLB. | Eur. J. Biochem. 271 4685-4695 2004 FEBS 2004 doi The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB Evgeny Vinogradov1 Catherine J. Paul2 Jianjun Li1 Yuchen Zhou2 Elizabeth A. Lyle3 Richard I. Tapping3 Andrew M. Kropinski2 and Malcolm B. Perry1 Institute for Biological Sciences National Research Council Ottawa ON Canada 2Queeris University Kingston ON Canada 3 University of Illinois Urbana IL USA In an attempt to isolate lipopolysaccharide from Spirochaeta aurantia Darveau-Hancock extraction of the cell mass was performed. While no lipopolysaccharide was found two carbohydrate-containing compounds were detected. They were resolved by size-exclusion chromatography into high molecular mass LGLA and low molecular mass LGLB fractions. Here we present the results of the analysis of the glycolipid LGLB. Deacylation of LGLB with hydrazine and separation of the products by using anion-exchange chromatography gave two major products. Their structure was determined by using chemical methods NMR and mass spectrometry. All monosaccharides had the D-configuration and aspartic acid had the L-configuration. Intact LGLB contained two fatty groups at O-2 and O-3 of the glycerol residue. Nonhydroxylated C14 to C18 fatty acids were identified which were predominantly unsaturated or branched. LGLb was able to gel Limulus amebocyte lysate albeit at a lower level than that observed for Escherichia coli O113 lipopolysaccharide. However even large amounts of LGLB were unable to stimulate any Toll-like receptor TLR examined including TLR4 and TLR2 previously shown to be sensitive to lipopolysaccharide and glycolipids from diverse bacterial origins including other spirochetes. Keywords glycolipid Spirochaeta aurantia structure. Spirochetes are a group of bacteria unified by spiral or flattened-waveform cell morphology and periplasmic endoflagella Spirochaeta is one of the six genera within this phylum 1 .

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