TAILIEUCHUNG - Báo cáo khoa học: Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis

The polar glycolipids were isolated from the thermophilic bacteriaMeiothermus taiwanensisATCC BAA-400 by eth-anol extraction and purified by Sephadex LH-20 and silica gel column chromatography. The fatty acid composition of O-acyl groups in the glycolipids was obtained by gas chro-matography mass spectroscopy analysis on their methyl esters derived from methanolysis and was made mainly of C15:0() andC17:0() fattyacids,withthemajority as branched fatty acids (over 80%). | Eur. J. Biochem. 271 4545-4551 2004 FEBS 2004 doi Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanensis Feng-Ling Yang1 Chun-Ping Lu1 Chien-Sheng Chen2 Mao-Yen Chen3 Hung-Liang Hsiao4 Yeu Su4 San-San Tsay3 Wei Zou5 and Shih-Hsiung Wu1 Institute of Biological Chemistry Academia Sinica Taipei Taiwan 2Department of Chemistry and 3Department of Life Science and Institute of Plant Biology National Taiwan University Taipei Taiwan 4Institute of Pharmacology College of Life Science National Yang-Ming University Shih-Pai Taipei Taiwan 5Institute for Biological Sciences National Research Council of Canada Ottawa Ontario Canada The polar glycolipids were isolated from the thermophilic bacteria Meiothermus taiwanensis ATCC BAA-400 by ethanol extraction and purified by Sephadex LH-20 and silica gel column chromatography. The fatty acid composition of O-acyl groups in the glycolipids was obtained by gas chromatography mass spectroscopy analysis on their methyl esters derived from methanolysis and was made mainly of C15 0 and C17 0 fatty acids with the majority as branched fatty acids over 80 . Removal of O-acyl groups under mild basic conditions provided two glycolipids which differ only in N-acyl substitution on a hexosamine. Electrospray mass spectroscopy analysis revealed that one has a C17 0 N-acyl group and the other hydroxy C17 0 in a ratio of about 1 . Furthermore complete de-lipidation with strong base followed by selective N-acetylation resulted in a homogeneous tetraglycosyl glycerol. The linkages and configurations of the carbohydrate moiety were then elucidated by MS and various NMR analyses. Thus the major glycolipid from M. taiwanensis ATCC BAA-400 was determined with the following structure a-Galp 1-6 -b-Galp 1-6 -b-GalNAcyl 1 2 -a-Glc 1 1 -Gro diester where N-acyl is C17 0 or hydroxy C17 0 fatty acid and the glycerol esters were mainly iso- and anteisobranched C15

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