TAILIEUCHUNG - Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein

Chicken metallothionein (ckMT) is the paradigm for the study of metallo-thioneins (MTs) in the Aves class of vertebrates. Available literature data depictckMTas a one-copy gene, encoding an MT protein highly similar to mammalian MT1. In contrast, the MT system in mammals consists of a four-member family exhibiting functional differentiation. | ềFEBS Journal Comparative metal binding and genomic analysis of the avian chicken and mammalian metallothionein Laura Villarreal1 Laura Tie2 Merce Capdevila1 and Silvia Atrian2 1 Departament de Quimica Facultat de Ciencies Universitat Autonoma de Barcelona Bellaterra Spain 2 Departament de Genetica Facultat de Biologia Universitat de Barcelona Spain Keywords dimerization Gallus gallus metal binding metallothionein molecular evolution Correspondence S. Atrian Department of Genetics Faculty of Biology University of Barcelona Avenue Diagonal645 08028-Barcelona Spain Fax 34 934034420 Tel 34 934021501 E-mail satrian@ Note L. Villarrealand L. Tio made equalcontributions to this work. Received 20 October 2005 revised 1 December 2005 accepted 2 December 2005 doi Chicken metallothionein ckMT is the paradigm for the study of metallo-thioneins MTs in the Aves class of vertebrates. Available literature data depict ckMT as a one-copy gene encoding an MT protein highly similar to mammalian MT1. In contrast the MT system in mammals consists of a four-member family exhibiting functional differentiation. This scenario prompted us to analyse the apparently distinct evolutionary patterns followed by MTs in birds and mammals at both the functional and structural levels. Thus in this work the ckMT metal binding abilities towards Zn II Cd II and Cu I have been thoroughly revisited and then compared with those of the mammalian MT1 and MT4 isoforms identified as zinc- and copper-thioneins respectively. Interestingly a new mechanism of MT dimerization is reported on the basis of the coordinating capacity of the ckMT C-terminal histidine. Furthermore an evolutionary study has been performed by means of in silico analyses of avian MT genes and proteins. The joint consideration of the functional and genomic data obtained questions the two features until now defining the avian MT system. Overall in vivo and in vitro metal-binding results reveal that the .

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