TAILIEUCHUNG - Báo cáo khoa học: Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni

N-Glycans from glycoproteins of the worm stage of the human parasite Schistosoma mansoniwere enzymatically released, fluorescently labelled and analysed using various mass spectrometric and chromatographic methods. A family of 28 mainly core-a1–6-fucosylated, diantennary N-glycans of composition Hex3)4HexNAc6)12Fuc1)6 was found to carry dimers ofN,N¢-diacetyllactosediamine [LacdiNAc or LDN; | ềFEBS Journal Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni Manfred Wuhrer Carolien A. M. Koeleman Andre M. Deelder and Cornells H. Hokke Biomolecular Mass Spectrometry Unit Department of Parasitology Center of Infectious Diseases Leiden University MedicalCenter The Netherlands Keywords mass spectrometry parasite terminal N-acetylgalactosamine trematode Correspondence M. Wuhrer Department of Parasitology Leiden University MedicalCenter . Box 9600 2300 RC Leiden the Netherlands Fax 31 71 526 6907 Tel 31 71 526 5077 E-mail Received 15 September 2005 revised 11 November 2005 accepted 21 November 2005 doi N-Glycans from glycoproteins of the worm stage of the human parasite Schistosoma mansoni were enzymatically released fluorescently labelled and analysed using various mass spectrometric and chromatographic methods. A family of 28 mainly core-a1-6-fucosylated diantennary N-glycans of composition Hex3_4HexNAc6_12Fuc1_6 was found to carry dimers of N N -diacetyllactosediamine LacdiNAc or LDN GalNAc P1-4 GlcNAc P1- with or without fucose a1-3-linked to the N-acetylglucosamine residues in the antennae GalNAc P1-4 Fuc a1-3 GlcNAc P1-3 GalNAc P1-4 Fuc a1-3 GlcNAc P1- . To date oligomeric LDN and oligomeric fucosylated LDN LDNF have been found only on N-glycans from mammalian cells engineered to express Caenorhabditis elegans p4-GalNAc transferase and human a3-fucosyltransferase IX Z. S. Kawar et al. 2005 J Biol Chem 280 12810-12819 . It now appears that LDN F repeats can also occur in a natural system such as the schistosome parasite. Like monomeric LDN and LDNF the dimeric LDN F moieties found here are expected to be targets of humoral and cellular immune responses during schistosome infection. The most common complex-type N-glycans of mammals contain N-acetyllactosamine-type antennae Lac-NAc Gal P1-4 GlcNAc P1- which can be substituted in various positions by fucoses

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