TAILIEUCHUNG - Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis

Jumonji (Jmj) is a transcriptional repressor that plays important roles in the suppression of cell proliferation and development of various tissues in the mouse. To further clarify the roles of Jmj during development and gain insight into mechanisms of Jmj-mediated transcriptional regulation, we have taken advantage ofDrosophilaas a model organism. | ỊFEBS Journal The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis Nobuhiro Sasai1 2 3 Yasuko Kato2 3 Gaku Kimura2 3 Takashi Takeuchi4 and Masamitsu Yamaguchi2 3 1 Venture Laboratory Kyoto Institute of Technology Japan 2 Department of Applied Biology Kyoto Institute of Technology Japan 3 Insect BiomedicalResearch Center Kyoto Institute of Technology Japan 4 Mitsubishi Kagaku Institute of Life Sciences MITILS Machida Japan Keywords euchromatin JmjC domain metamorphosis suppressor of PEV transcriptionalsilencing Correspondence M. Yamaguchi Department of Applied Biology Kyoto Institute of Technology Matsugasaki Sakyo-ku Kyoto 606-8585 Japan Fax 81 75 724 7760 Tel 81 75 724 7781 E-mail myamaguc@ Present address CNRS UMR218 Institute Curie Paris France Received 25 July 2007 revised 4 October 2007 accepted 10 October 2007 doi Jumonji Jmj is a transcriptional repressor that plays important roles in the suppression of cell proliferation and development of various tissues in the mouse. To further clarify the roles of Jmj during development and gain insight into mechanisms of Jmj-mediated transcriptional regulation we have taken advantage of Drosophila as a model organism. Drosophila Jmj dJmj shares high homology with mammalian Jmj in the JmjN JmjC and AT-rich interaction domains as well as in the N-terminal repression domain. dJmj localizes to hundreds of euchromatic sites but not to chromocenter heterochromatin on salivary gland polytene chromosomes. In addition dJmj is excluded from regions stained with an antibody against Ser5-phosphorylated RNA polymerase II suggesting a function of dJmj in transcriptionally inactive chromatin. Loss of djmj results in larval and pupal lethality with phenotypes similar to those observed in mutants of ecdysone-regulated genes implying the involvement of dJmj in the repression of gene expression in the ecdysone pathway. Transgenic mouse Jmj mostly

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