TAILIEUCHUNG - Báo cáo khoa học: Adaptation to high temperatures through macromolecular dynamics by neutron scattering

Work on the relationship between hyperthermophile protein dynamics, sta-bility and activity is reviewed. Neutron spectroscopy has been applied to measure and compare the macromolecular dynamics of various hyper-thermophilic and mesophilic proteins, under different conditions. | MINIREVIEW Adaptation to high temperatures through macromolecular dynamics by neutron scattering Moeava Tehei1 and Giuseppe Zaccai1 2 1 Institut Laue-Langevin Grenoble France 2 Institut de Biologie Structurale Laboratoire de Biophysique Moleculaire Grenoble France Keywords experimentalmolecular dynamics flexibility rigidity neutron scattering thermostability Correspondence T. Moeava Institut Laue-Langevin 6 rue Jules Horowitz BP 156 38042 Grenoble Cedex 9 France Fax 33 0 47 6207688 Tel 33 0 47 6207738 E-mail v-tehei@ Received 28 February 2007 revised 25 May 2007 accepted 22 June 2007 doi Work on the relationship between hyperthermophile protein dynamics stability and activity is reviewed. Neutron spectroscopy has been applied to measure and compare the macromolecular dynamics of various hyper-thermophilic and mesophilic proteins under different conditions. First molecular dynamics have been analyzed for the hyperthermophile malate dehydrogenase from Methanococcus jannaschii and a mesophilic homologue the lactate dehydrogenase from Oryctolagus cunniculus rabbit muscle. The neutron scattering approach has provided independent measurements of the global flexibility and structural resilience of each protein and it has been demonstrated that macromolecular dynamics represents one of the molecular mechanisms of thermoadaptation. The resilience was found to be higher for the hyperthermophilic protein thus ensuring similar flexibilities in both enzymes at their optimal activity temperature. Second the neutron method has been developed to quantify the average macromolecular flexibility and resilience within the natural crowded environment of the cell and mean macromolecular motions have been measured in vivo in psychrophile mesophile thermophile and hyperthermophile bacteria. The macromolecular resilience in bacteria was found to increase with adaptation to high temperatures whereas flexibility was maintained within narrow limits .

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