TAILIEUCHUNG - Evaluation of in-house PCR for diagnosis of smear-negative pulmonary tuberculosis in Kampala, Uganda

Nucleic acid amplification tests (NAATs) have offered hope for rapid diagnosis of tuberculosis (TB). However, their efficiency with smear-negative samples has not been widely studied in low income settings. Here, we evaluated in-house PCR assay for diagnosis of smear-negative TB using Lowenstein-Jensen (LJ) culture as the baseline test. Two hundred and five pulmonary TB (PTB) suspects with smear-negative sputum samples, admitted on a short stay emergency ward at Mulago Hospital in Kampala, Uganda, were enrolled. Two smear-negative sputum samples were obtained from each PTB suspect and processed simultaneously for identification of MTBC using in-house PCR and LJ culture | Nakiyingi et al. BMC Research Notes 2012 5 487 http 1756-0500 5 487 BMC Research Notes RESEARCH ARTICLE Open Access Evaluation of in-house PCR for diagnosis of smear-negative pulmonary tuberculosis in Kampala Uganda I riitx l l x zi inni1 2 rAsix irì D IZ-it-oot-Q3 Or amd 2 yA illi rvì yA mrmrìrisi3 Ir icotpf i R QiTm7i 1 Lydid Ndkiyingi Ddvid P Kdteete Ponsidiio ucdmd vviiiidiII vvuiuuiid Joseph B jempd Benon B Asiimwe3 Fred A Kdtdbdzi3 Achilles Kdtdmbd2 Laurence Huang4 Moses L Jolobd3 and Hdrriet Mdydnjd-Kizzd2 Abstract Background Nucleic dcid dmplificdtion tests NAATs hdve offered hope for rdpid didgnosis of tuberculosis TB . However their efficiency with smedr-negdtive sdmples hds not been widely studied in low income settings. Here we evdludted in-house PCR dssdy for didgnosis of smedr-negdtive TB using Lowenstein-Jensen LJ culture ds the bdseline test. Two hundred dnd five pulmondry TB PTB suspects with smedr-negdtive sputum sdmples ddmitted on d short stdy emergency wdrd dt Muldgo Hospitdl in Kdmpdld Ugdndd were enrolled. Two smedr-negdtive sputum sdmples were obtdined from edch PTB suspect dnd processed simultdneously for identificdtion of MTBC using in-house PCR dnd LJ culture. Results Seventy two PTB suspects 35 72 205 were LJ culture positive while 128 128 205 were PCR-positive. The sensitivity dnd specificity of in-house PCR for didgnosis of smedr-negdtive PTB were 75 95 CI dnd 95 CI respectively. The positive dnd negdtive predictive vdlues were 39 95 CI dnd 95 CI respectively while the positive dnd negdtive likelihood rdtios were 95 CI dnd 95 CI respectively. One hundred dnd seventeen LJ culture-negdtive suspects 75 PCR-positive dnd 42 PCR-negdtive were enrolled for follow-up dt 2 months. Of the PCR-positive suspects 45 60 45 75 were still dlive of whom 29 29 45 returned for the follow-up visit 15 20 15 75 suspects died while dnother 15 20 15

• Y học thường thức
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Skim milk powder
• Spray drying
• Crumble feed
• Spray dried probiotics
• Crumble feed during storage
• Red dragon fruit
• Freeze dried
• Lactobacillus delbrueckii subsp
• Streptococcus thermophilus
• Dehydrated yogurt
• Pros and Con