TAILIEUCHUNG - báo cáo khoa hoc : Effects of phosphatidylethanolamine N-methyltransferase on phospholipid composition, microvillus formation and bile salt resistance in LLC-PK1 cells

Bile salts are potent detergents and can disrupt cellular membranes, which causes cholestasis and hepatocellular injury. However, the mechanism for the resistance of the canalicular membrane against bile salts is not clear. Phosphatidylethanolamine (PE) is converted to phosphatidylcholine (PC) in the liver by phosphatidylethanolamine N-methyltransferase (PEMT). | IFEBS Journal Effects of phosphatidylethanolamine N-methyltransferase on phospholipid composition microvillus formation and bile salt resistance in LLC-PK1 cells 2 2 2 2 Shin-ya Morita Noriaki Ikeda Manami Horikami Kuriko Soda Kosuke Ishihara Reiko Teraoka2 Tomohiro Terada1 and Shuji Kitagawa2 1 Department of Pharmacy Shiga University of MedicalScience Hospital Otsu City Shiga Japan 2 Kobe PharmaceuticalUniversity Kobe Japan Keywords bile salt microvillus phosphatidylcholine phosphatidylethanolamine N-methyltransferase sphingomyelin Correspondence . Morita Department of Pharmacy Shiga University of MedicalScience Hospital Otsu City Shiga 520-2192 Japan Fax 81 77 548 2681 Tel 81 77 548 2681 E-mail smorita@ Received 30 May 2011 revised 23 August 2011 accepted 27 September 2011 doi Bile salts are potent detergents and can disrupt cellular membranes which causes cholestasis and hepatocellular injury. However the mechanism for the resistance of the canalicular membrane against bile salts is not clear. Phosphatidylethanolamine PE is converted to phosphatidylcholine PC in the liver by phosphatidylethanolamine N-methyltransferase PEMT . In this study to investigate the effect of PEMT expression on the resistance to bile salts we established an LLC-PK1 cell line stably expressing PEMT. By using enzymatic assays we showed that the expression of PEMT increased the cellular PC content lowered the PE content but had no effect on the sphingomyelin content. Consequently PEMT expression led to reductions in PE PC and sphingomyelin PC ratios. Mass spectrometry demonstrated that PEMT expression increased the levels of PC species containing longer acyl chains and almost all ether-linked PC species. PEMT expression enhanced the resistance to duramycin and lysenin suggesting decreased ratios of PE and sphingomyelin in the apical membrane respectively. In addition SEM revealed that PEMT expression increased the diameter of

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• N methyltransferase
• medicine
• oxidation
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes