TAILIEUCHUNG - Báo cáo khoa học: A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase

A new highly cytotoxic protein, toxophallin, was recently isolated from the fruit body of the death cap Amanita phalloidesmushroom [Stasyket al. (2008) Studia Biologica2, 21–32]. The physico-chemical, chemical and bio-logical characteristics of toxophallin differ distinctly from those of another death cap toxic protein, namely phallolysin. | A new highly toxic protein isolated from the death cap Amanita phalloides is an L-amino acid oxidase Taras Stasyk1 2 Maxim Lutsik-Kordovsky1 Christer Wernstedt3 Volodymyr Antonyuk1 Olga Klyuchivska1 Serhiy Souchelnytskyi4 Ulf Hellman3 and Rostyslav Stoika1 1 Institute of CellBiology NationalAcademy of Sciences of Ukraine Lviv Ukraine 2 Biocenter Innsbruck MedicalUniversity Austria 3 Ludwig Institute for Cancer Research Ltd Uppsala University Sweden 4 Karolinska Biomics Center Karolinska Institutet Stockholm Sweden Keywords Amanita phalloides apoptosis death cap L-amino acid oxidase toxic protein Correspondence R. Stoika Institute of CellBiology National Academy of Sciences of Ukraine Drahomanov Street 14 16 79005 Lviv Ukraine Fax 38 032 261 22 87 Tel 38 032 261 22 87 E-mail stoika@ Database Nucleotide sequence data have been submitted to the GenBank database under the accession number GU220069 Received 8 October 2009 revised 2 December 2009 accepted 21 December 2009 doi A new highly cytotoxic protein toxophallin was recently isolated from the fruit body of the death cap Amanita phalloides mushroom Stasyk et al. 2008 Studia Biologica 2 21-32 . The physico-chemical chemical and biological characteristics of toxophallin differ distinctly from those of another death cap toxic protein namely phallolysin. The interaction of toxophallin with target cells is not mediated by a specific cell surface receptor. It induces chromatin condensation as well as DNA and nucleus fragmentation which are typical for apoptosis. However caspase III inhibitor ben-zyloxycarbonyl-Asp OMe -fluoromethylketone did not stop toxophallin-induced DNA fragmentation. Thus toxophallin uses a caspase-independent pathway of apoptosis induction. In the present study we applied a complementary approach based on a combination of proteomics and molecular biology tools for the protein identification of toxophallin. The primary structure of toxophallin was .

• Báo cáo khoa học
• Scientific reports
• scientific research
• biochemistry
• oxidase
• tumor cells
• Structural Biology
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes