TAILIEUCHUNG - Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines

From within the small yet growing body of research focused upon CAM use for pregnancy [2,5], the majority has examined pregnant women’s use of discrete supplements or treatments and, despite recommendations [23,24], there has been little exploration of women’s consultations with CAM practitioners. One exception is a recent longitudinal cohort study (n=535) [7] which identified no significant change in this prevalence rate over a 10 year period compared with non-pregnant women. Unfortunately, this recent work does not discern the prevalence of pregnant women’s use of specific types of CAM practitioners or examine the patterns of pregnant women’s consultations with. | Schropfer et al. BMC Cancer 2010 10 553 http 1471-2407 10 553 BMC Cancer RESEARCH ARTICLE Open Access Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines Andrea Schropfer Ulrike Kammerer Michaela Kapp Johannes Dietl Sonja Feix Jelena Anacker Abstract Background Matrix metalloproteinases MMPs are involved in the degradation of protein components of the extracellular matrix and thus play an important role in tumor invasion and metastasis. Their expression is related to the progression of gynecological cancers . endometrial cervical or ovarian carcinoma . In this study we investigated the expression pattern of the 23 MMPs currently known in humans in different gynecological cancer cell lines. Methods In total cell lines from three endometrium carcinomas Ishikawa HEC-1-A AN3 CA three cervical carcinomas HeLa Caski SiHa three chorioncarcinomas JEG JAR BeWo two ovarian cancers BG-1 OAW-42 and one teratocarcinoma PA-1 were examined. The expression of MMPs was analyzed by RT-PCR Western blot and gelatin zymography. Results We demonstrated that the cell lines examined can constitutively express a wide variety of MMPs on mRNA and protein level. While MMP-2 -11 -14 and -24 were widely expressed no expression was seen for MMP-12 -16 -20 -25 -26 -27 in any of the cell lines. A broad range of 16 MMPs could be found in the PA1 cells and thus this cell line could be used as a positive control for general MMP experiments. While the three cervical cancer cell lines expressed 10-14 different MMPs the median expression in endometrial and choriocarcinoma cells was 7 different enzymes. The two investigated ovarian cancer cell lines showed a distinctive difference in the number of expressed MMPs 2 vs. 10 . Conclusions Ishikawa Caski OAW-42 and BeWo cell lines could be the best choice for all future experiments on MMP regulation and their role in endometrial cervical ovarian or choriocarcinoma development whereas the .

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