TAILIEUCHUNG - Báo cáo khoa hoc:"Comparative analysis on the structural features of the 5 flanking region of κ-casein genes from six different species"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Comparative analysis on the structural features of the 5 flanking region of κ-casein genes from six different species | 117 Genet Sei. Evol. 34 2002 117-128 INRA EDP Sciences 2002 DOI gse 2001007 Original article Comparative analysis on the structural features of the 50 flanking region of k-casein genes from six different species Ákos Gerencséra Endre Bartaa Simon Boab Petros Kastanisb Zsuzsanna Boszea c. Bruce A. Whitelawb a Department of Animal Biology Agricultural Biotechnology Center 2100 Gõdõllõ Szent-Gyorgyi A. Hungary b Department of Gene Expression and Development Roslin Institute Edinburgh Roslin Midlothian EH25-9PS Scotland UK Received 20 February 2001 accepted 1st August 2001 Abstract - k-casein plays an essential role in the formation stabilisation and aggregation of milk micelles. Control of k-casein expression reflects this essential role although an understanding of the mechanisms involved lags behind that of the other milk protein genes. We determined the 5 -flanking sequences for the murine rabbit and human k-casein genes and compared them to the published ruminant sequences. The most conserved region was not the proximal promoter region but an approximately 400 bp long region centred 800 bp upstream of the TATA box. This region contained two highly conserved MGF STAT5 sites with common spacing relative to each other. In this region six conserved short stretches of similarity were also found which did not correspond to known transcription factor consensus sites. On the contrary to ruminant and human 5 regulatory sequences the rabbit and murine 5 -flanking regions did not harbour any kind of repetitive elements. We generated a phylogenetic tree of the six species based on multiple alignment of the k-casein sequences. This study identified conserved candidate transcriptional regulatory elements within the k-casein gene promoter. K-casein 5 regulatory region transcription factor binding sites repetitive elements 1. introduction Although milk casein composition varies consideraBly between livestock species k-casein seems to Be ubiquitous in accordance with

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