TAILIEUCHUNG - Báo cáo khoa học: The in vitro nuclear aggregates of polyamines

Natural polyamines (putrescine, spermidine, and spermine) self-assemble in a simulated physiological environment (50 mmsodium phosphate buffer, pH ), generating in vitro nuclear aggregates of polyamines (ivNAPs). These supramolecular compounds are similar in structure and molecular mass to naturally occurring cellular nuclear aggregates of polyamines, and they share the ability of NAPs to interact with and protect the genomic DNA against nuclease degradation. | The in vitro nuclear aggregates of polyamines Aldo Di Luccia1 2 Gianluca Picariello1 Giuseppe Iacomino1 Annarita Formisano1 Luigi Paduano3 and Luciano D Agostino1 4 1 Institute of Food Sciences NationalResearch Council CNR Avellino Italy 2 Department PROGESA University of Bari Italy 3 Department of Chemistry University of Naples Federico II Italy 4 Department of Clinicaland ExperimentalMedicine University of Naples Federico II Italy Keywords DNA interactions nanostructures polyamines self-assembly supramolecular chemistry Correspondence L. D Agostino Department of Clinicaland ExperimentalMedicine University of Naples Federico II Ed. 6 Via S. Pansini 5 80131 Naples Italy Fax 39 081 7462707 Tel 39 081 7462707 E-mail luciano@ These authors contributed equally to this work Received 16 September 2008 revised 9 February 2009 accepted 11 February 2009 doi Natural polyamines putrescine spermidine and spermine self-assemble in a simulated physiological environment 50 mM sodium phosphate buffer pH generating in vitro nuclear aggregates of polyamines ivNAPs . These supramolecular compounds are similar in structure and molecular mass to naturally occurring cellular nuclear aggregates of polyamines and they share the ability of NAPs to interact with and protect the genomic DNA against nuclease degradation. Three main ivNAP compounds were separated by gel permeation chromatography. Their elution was carried out with 50 mM sodium phosphate buffer supplemented with 150 mM NaCl. Freezing and thawing of selected chromatographic fractions obtained by GPC runs in which the mobile phase was sodium phosphate buffer not supplemented with NaCl yielded three different microcrystallites specifically corresponding to the ivNAPs all of which were able to bind DNA. In this study we demonstrated that in vitro self-assembly of polyamines and phosphates is a spontaneous reproducible and inexpensive event and provided the indications for the production

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