TAILIEUCHUNG - Báo cáo lâm nghiệp: "The use of silica sand in micropropagation of woods"

Tuyển tập những bài báo cáo nghiên cứu khoa học hay nhất được đăng trên tạp chí JOURNAL OF FOREST SCIENCE đề tài: The use of silica sand in micropropagation of woods. | JOURNAL OF FOREST SCIENCE 53 2007 2 88-92 The use of silica sand in micropropagation of woods H. Prknová Faculty of Forestry and Environment Czech University of Life Sciences in Prague Prague Czech Republic ABSTRACT Cultures in vitro made in agar are rather precarious because gel strength varies both with the medium formula used and the source and grade of agar. Any solidifying agent like for example agar should be strong enough to support cultivated plantlets yet liquid enough to allow the nutrients and drossy products from plants through the medium. It should also be a chemically inert material. Agar especially in acid solutions is an undefined constituent of culture media namely in the mentioned properties. Silica sand used in cultures of herbs up to the present time is applicable also in cultures of Sorbus sudetica. The required acid medium is exactly defined if sand is substituted for agar. Similar cultures of wood species including conifers will be realized in future research. Keywords micropropagation agar silica sand acid medium Sorbus sudetica Agar is used for the micropropagation of wood species in vitro as a support whereby nutrient media are solidified. Agar is used in 6-8 g l concentration to be solid enough to support cultivated plantlets yet liquid enough to allow the nutrients and drossy products from plants to pass through the medium. Agar a natural polysaccharide containing D- and L-galactose partly esterified by sulphuric acid has however a disability range. It is considered a chemically undefined substance which is why media with agar are just partially defined. Properties like solidity and adulterants vary in origin and by the technique of its factory processing. Agar gel becomes liquid during sterilization in an autoclave as a result of hydrolysis namely more in media with higher content of salts and phytohormones than in less concentrated media. It is also more unstable with pH than by pH . The liquidity then has an impact on the .

• Báo cáo khoa học
• Báo cáo lâm nghiệp hay
• cách trình bày báo cáo
• báo cáo lâm nghiệp
• công trình nghiên cứu lâm nghiệp
• tài liệu về lâm nghiệp