TAILIEUCHUNG - Báo cáo sinh học: " Molecular evolution of the leptin exon 3 in some species of the family Canidae"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Molecular evolution of the leptin exon 3 in some species of the family Canidae | 573 Genet Sei. Evol. 35 2003 573-580 INRA EDP Sciences 2003 DOI gse 2003040 Original article Molecular evolution of the leptin exon 3 in some species of the family Canidae Agata Chmurzynskaa Magdalena Zajaca b Marek Switonskia a Department of Genetics and Animal Breeding AuGUst Cieszkowski Agricultural University of Poznan Poland b Institute of Human Genetics Polish Academy of Sciences Poznan Poland Received 9 September 2002 accepted 5 February 2003 Abstract - The structure of the leptin gene seems to be well conserved. The polymorphism of this gene in four species belonging to the Canidae family the dog Canis familiaris - 16 different breeds the Chinese racoon dog Nyctereutes procyonoides procyonoides the red fox Vulpes vulpes and the arctic fox Alopex lagopus were studied with the use of single strand conformation polymorphism SSCP restriction fragment length polymorphism RFLP and DNA sequencing techniques. For exon 2 all species presented the same SSCP pattern while in exon 3 some differences were found. DNA sequencing of exon 3 revealed the presence of six nucleotide substitutions differentiating the studied species. Three of them cause amino acid substitutions as well. For all dog breeds studied SSCP patterns were identical. leptin Canidae polymorphism 1. introduction The Canidae family consists of 36 species and can be divided into three groups the red fox-like canids . the red and Arctic fox the South American foxes . pampas fox and the wolf-like canids. The species such as the racoon dog and the bat-eared fox are monotypic genera and represent distinct lineages 21 . The racoon dog has the most primitive chromosome set including many ancestral segments and additionally some blocks are homologous to those in cats 14 . The chromosome number within the Canidae family ranges from 34 B chromosomes in the red fox to 78 in the dog wolf and jackal. The most phenotypically diverse canid is the domestic dog Canis familiaris 20 . The high degree of .

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