TAILIEUCHUNG - Báo cáo y học: " Influenza A viral loads in respiratory samples collected from patients infected with pandemic H1N1, seasonal H1N1 and H3N2 viruses"

Influenza A viral loads in respiratory samples collected from patients infected with pandemic H1N1, seasonal H1N1 and H3N2 viruses | Ngaosuwankul et al. Virology Journal 2010 7 75 http content 7 1 75 VIROLOGY JOURNAL RESEARCH Open Access Influenza A viral loads in respiratory samples collected from patients infected with pandemic H1N1 seasonal H1N1 and H3N2 viruses Nathamon Ngaosuwankul 1 Pirom Noisumdaeng1 Pisut Komolsiri1 Phisanu Pooruk1 Kulkanya Chokephaibulkit2 Tawee Chotpitayasunondh3 Chariya Sangsajja4 Charoen Chuchottaworn5 Jeremy Farrar6 and Pilaipan Puthavathana 1 Abstract Background Nasopharyngeal aspirate NPA nasal swab NS and throat swab TS are common specimens used for diagnosis of respiratory virus infections based on the detection of viral genomes viral antigens and viral isolation. However there is no documented data regarding the type of specimen that yields the best result of viral detection. In this study quantitative real time RT-PCR specific for M gene was used to determine influenza A viral loads present in NS NPA and TS samples collected from patients infected with the 2009 pandemic H1N1 seasonal H1N1 and H3N2 viruses. Various copy numbers of RNA transcripts derived from recombinant plasmids containing complete M gene insert of each virus strain were assayed by RT-PCR. A standard curve for viral RNA quantification was constructed by plotting each Ct value against the log quantity of each standard RNA copy number. Results Copy numbers of M gene were obtained through the extrapolation of Ct values of the test samples against the corresponding standard curve. Among a total of 29 patients with severe influenza enrolled in this study 12 cases of the 2009 pandemic influenza 5 cases of seasonal H1N1 and 12 cases of seasonal H3N2 virus NPA was found to contain significantly highest amount of viral loads and followed in order by NS and TS specimen. Viral loads among patients infected with those viruses were comparable regarding type of specimen analyzed. Conclusion Based on M gene copy numbers we conclude that NPA is the best specimen for detection of influenza A

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