TAILIEUCHUNG - Analysis of genetic polymorphism in genes encoding cathelicidins from vietnam indigenous yellow cattle breeds

Cathelicidins include antimicrobial peptides (AMPs) and are involved in the innate immune defense against infections in mammals. Polymorphisms in the DNA sequence of cathelicidin genes could be relevant to inherited variations of host innate immunity, adaptation, and pathogen resistance. This study aims to investigate the sequence polymorphism of cathelicidin genes including CATHL2 and CATHL4 from local indigenous yellow cattle breeds of Vietnam. | ACADEMIA JOURNAL OF BIOLOGY 2023 45 3 23 33 DOI 2615-9023 18295 ANALYSIS OF GENETIC POLYMORPHISM IN GENES ENCODING CATHELICIDINS FROM VIETNAM INDIGENOUS YELLOW CATTLE BREEDS Thi Tuoi Do1 Hong Ngoc Ta1 Thi Thuy Anh Tran1 Doan Lan Pham2 Thi Phuc Do1 Thi Hong Van Nguyen1 1 VNU University of Science Vietnam National University Hanoi 334 Nguyen Trai Ha Noi Vietnam 2 National Institute of Animal Science 9 Tan Phong Ha Noi Vietnam Received 24 April 2023 accepted 18 September 2023 ABSTRACT Cathelicidins include antimicrobial peptides AMPs and are involved in the innate immune defense against infections in mammals. Polymorphisms in the DNA sequence of cathelicidin genes could be relevant to inherited variations of host innate immunity adaptation and pathogen resistance. This study aims to investigate the sequence polymorphism of cathelicidin genes including CATHL2 and CATHL4 from local indigenous yellow cattle breeds of Vietnam. Genomic DNA samples were extracted from 52 individuals collected from different cattle populations in Vietnam including Ha Giang Thanh Hoa Nghe An and Phu Yen. The CATHL2 and CATHL4 genes were amplified by PCR following sequencing for identification of the single nucleotide polymorphisms SNPs and or insertion-deletion indel DNA sequence variations. The copy number variations CNVs of the CATHL4 gene were determined by cloning single strand conformation polymorphism SSCP and real-time PCR. The comparative analysis results showed that there are 13 SNPs detected in all sequences of CATHL2 gene including 9 SNPs in intron 2 and intron 3 and 4 missense substitutions in exon 1 2 and 3. These SNPs do not affect the predicted tertiary structure of Bac5 encoded by this gene. For CATHL4 the results revealed 15 SNPs 3 indels and 1 repeat variation as motif TG n G n AC n C n. Among these variations the deletion 46delC in exon 1 of CATHL4 was detected in a sample of the Ha Giang breed causing a truncated polypeptide as predicted. The average copy number

• Sinh học
• Genetic polymorphism
• Vietnam indigenous cattle
• Antimicrobial peptides
• Single strand conformation polymorphism
• Animal science
• Wild relatives of wheat
• Genetic polymorphism in wheat
• Wild relatives using ISSR markers
• ISSR markers
• Sodium nitrite
• Assessment of genetic polymorphism
• Sugarcane somaclones
• Random amplified polymorphic DNA
• ERα gene
• Genetic polymorphism of estrogen hormone receptor
• Estrogen hormone receptor
• Gene in buffaloes
• Genetic diversity
• RAPD markers
• Wheat (Triticum aestivum L ) genotypes
• RAPD polymorphism in wheat
• Genetic analysis
• Genetic improvement
• Sequence related amplified polymorphism
• Simple sequence repeat
• Genetic diversity of watermelon
• Candidate gene
• Genetic marker
• Polymorphism vis a vis immune response
• Important infectious diseases
• Genetic gain is cumulative
• Quantitative genetic variations
• Oryza sativa
• Grain protein content
• Rice and segregating lines
• Rice (Oryza sativa L )
• Parental Polymorphism Survey
• Amplified fragment length polymorphism
• Genetic structure
• Nuclear DNA content
• Genetic relationships
• Growth parameters
• Surti goats
• Growth parameters in surti goats
• Growth hormone gene polymorphism
• SNPs technique
• PCR – RFLP
• MHC class II DRB3
• Salem black goats by PCR RFLP
• Genetic resources
• Leaf antioxidant properties
• Molecular markers
• BMC Plant Biology
• Bread wheat
• Clusters of unique markers
• Genetic phylogeny
• Next generation sequencing
• Diverse parents
• Simple sequences repeat
• Rice germplasm using SSR markers
• Genetic diversity in rice
• Journal of Botany
• Crimean juniper
• Genetic isolation
• Intron targeted amplified polymorphism (ITAP)
• Molecular variance
• Simple sequence repeat (SSR)
• Matricaria chamomilla l
• Explores agro morphological
• Genetic variation affected by chemical mutagen
• Genetic improvment
• Polymorphism information content
• Mycobacterium tuberculosis
• Single nucleotide polymorphism
• AG85 genes
• 13 BCG strains
• BMC Pediatrics
• P glycoprotein
• Antide pressants
• Including antidepressant drugs
• C3435T polymorphisms
• BMC Cancer
• Vitamin D binding protein
• Colorectal cancer
• Dietary vitamin D
• Microsatellite instability
• BRAF mutations
• Breast cancer
• Genetic heritability
• Confidence interval
• BMC Musculoskeletal Disorders
• Intervertebral disk degeneration
• Meta analysis
• Genetic factors
• Starch content
• Bin map
• Genetic basis
• Maize kernel
• Milk Proteins
• Milk Production
• Lactogenic Hormones
• Quality Traits
• Fat Content
• Cross Goats
• Scald disease
• Principle component analysis
• Quantitative trait loci
• Neighbor joining
• Polymerase chain reaction
• Erzincan horticultural central research institute
• Hypericum perforatum
• IRAP markers
• Inter retrotransposon amplified polymorphism
• Principal coordinate analysis