TAILIEUCHUNG - Exosomes derived from human neural stem cells stimulated by interferon gamma improve therapeutic ability in ischemic stroke model

Transplanted neural stem cells promote neural tissue regeneration and functional recovery primarily by releasing paracrine factors. Exosomes act as important secreted paracrine molecules to deliver therapeutic agents involved in cellular functions. Here, we focused on the role of exosomes (hNSC-Exo) derived from human neural stem cells (hNSCs). We utilized the pro-inflammatory factor interferon gamma (IFN-c) to induce the generation of altered exosomes (IFN-c-hNSC-Exo), and compared their roles with those of hNSC-Exo and explored the potential mechanism. Importantly, IFN-c preconditioning did not affect the secretion, but significantly altered the ability of exosomes derived from hNSCs. Moreover, IFN-c-hNSC-Exo was functionally superior to hNSC-Exo; showed increased cell proliferation and cell survival and decreased cell apoptosis in vitro. Furthermore, IFN-c-hNSC-Exo further exerted therapeutic effects (showed better behavioral and structural outcomes) compared to those of hNSCs-Exo in an ischemic stroke rat model. Next-generation sequencing (NGS) revealed specific exosomal miRNAs (hsamiR-206, hsa-miR-133a-3p and hsa-miR-3656) in IFN-c-hNSC-Exo with important roles in cell survival. Thus, our findings demonstrate that the inflammatory factor IFN-c can regulate the functions of exosomes and highlight its role in regulating the application of neural stem cell-derived exosomes. | Exosomes derived from human neural stem cells stimulated by interferon gamma improve therapeutic ability in ischemic stroke model

• Y khoa - Dược
• Neural stem cells
• Interferon gamma
• Ischemic stroke
• Ischemic stroke model
• Human neural stem cells stimulated
• BMC Veterinary Research
• Multipotent mesenchymal stromal cells
• Mesenchymal stem cells
• Neurodegenerative diseases
• Neural cellular lineages
• BMC Anesthesiology
• Animal disease models
• Related microRNAs
• Intravenous anesthetic
• γ Aminobutyric acid
• Neurologic disorder
• Sevoflurane inhalation anesthesia
• Genome Biology
• Single cell RNAseq
• In silico lineage tracing
• Schmidtea mediterranea
• Transgenic labeling
• NSCs isolation
• Various neurodegenerative diseasesm
• Neural regeneration research
• Glioma stem cell
• Neural stem cell
• DNA mark changes
• Cell proliferation
• Neural crest
• Human dental pulp
• Cell based therapies
• Postnatal dental pulp stem cells
• Stemness maintenance
• Neural Stem
• Cell Heterogeneity
• Retinal Progenitors
• Pathogenetic Mechanisms
• CNS Diseases
• Nitric Oxide System
• Neural
• Cells
• Tao Sun
• Health
• Sciences
• medicine
• pharmacolocy
• BMC Molecular and Cell Biology
• RNA binding protein involved
• Gene expression
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