TAILIEUCHUNG - Plaque purification of bluetongue virus serotype-4 (BTV-4)

The present study was taken up with a view to undertake plaque purification of BTV-4 isolate available in the department, to confirm its purity. BTV-4 isolate was adapted to Vero cell line and had a titer of after 8 passages in the process of plaque purification which was determined as prior to adaption. Plaque purification was carried for three times using agarose overlaying method in 6 well plate. Each time plaques were observed from 4th day of infection of approximately not more than 1mm diameter. | 2018 7 4 2837-2844 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 7 Number 04 2018 Journal homepage http Original Research Article https Plaque Purification of Bluetongue Virus Serotype-4 BTV-4 M. Srikanth Reddy1 Kalyani Putty1 Y. Narasimha Reddy1 . Rao2 M. Ramakoti Reddy3 Uma2 Sunil R. Patil1 Susmitha Birru1 Abdul Muzeer Shaik1 and Dhanalakshmi Kancharlapally1 1College of Veterinary Science Rajendranagar Hyderabad-500030 Telangana India 2Ella Foundation Genome Valley Hyderabad-500 078 Telangana India directorate of Poultry Research Rajendranagar Hyderabad-500030 Telangana India Corresponding author ABSTRACT Keywords BTV-4 Vero cells TCID50 Plaque purification Sea plaque agarose RT-PCR Article Info Accepted 23 March 2018 Available Online 10 April 2018 The present study was taken up with a view to undertake plaque purification of BTV-4 isolate available in the department to confirm its purity. BTV-4 isolate was adapted to Vero cell line and had a titer of 106 46 ml after 8 passages in the process of plaque purification which was determined as ml prior to adaption. Plaque purification was carried for three times using agarose overlaying method in 6 well plate. Each time plaques were observed from 4th day of infection of approximately not more than 1mm diameter. One of the plaques from third round of purification was grown in T25 flask and the resultant cell culture fluid was used for RNA isolation and RT-PCR through which the respective plaque was confirmed as BTV-4. Therefore it was confirmed that there was no other serotype other than BTV-4 and the plaque purified virus can be used either to study pathogenesis or to raise hyperimmune serum. Introduction Bluetongue BT an infectious non-contagious viral disease of sheep and other domestic and wild ruminants caused by bluetongue virus BTV is a segmented .

• Sinh học
• Vero cells
• Plaque purification
• Sea plaque agarose
• RT PCR
• Bluetongue virus serotype 4
• Carbohydrate polymers
• Pectin derivative
• Antitumor property
• Caco 2 cells
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo
• tài liệu báo cáo nghiên cứu khoa học
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• báo cáo khoa học
• Pseudomonas aeruginosa
• Type III secretion system
• G protein activating protein
• ADP ribosylation