TAILIEUCHUNG - Cytotoxic activities of some Pseudomonas aeruginosa isolates: Possible mechanisms and approaches for inhibition

The cytotoxicity of 6 highly cytotoxic Pseudomonas aeruginosa isolates was tested against Vero cells. Cytotoxicity was found to be cell-associated, high cytotoxicity was observed at a multiplicity of infection of 200:1, and this activity gradually decreased as the bacterial count decreased. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 69-80 © TÜBİTAK doi: Cytotoxic activities of some Pseudomonas aeruginosa isolates: possible mechanisms and approaches for inhibition Ghadir Saeed EL-HOUSSEINY, Mohammad Mabrouk ABOULWAFA*, Nadia Abdelhaleim HASSOUNA Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt Received: Accepted: Published Online: Printed: Abstract: The cytotoxicity of 6 highly cytotoxic Pseudomonas aeruginosa isolates was tested against Vero cells. Cytotoxicity was found to be cell-associated, high cytotoxicity was observed at a multiplicity of infection of 200:1, and this activity gradually decreased as the bacterial count decreased. This cytotoxicity caused rounding and detachment of the Vero monolayer as early as 1 h after infection. Moreover, viable metabolically active bacteria were necessary for Vero cell killing. These results imply that one or more of the type III secretion system effector proteins (ExoU, ExoS, ExoT, or ExoY) were responsible for the observed cytotoxic effects. This cytotoxicity was affected by changes in pH and temperature and was associated with high adherence but poor invasion levels. The isolates P127, P139, P205, and P217 exhibited their cytotoxic effect within short infection periods (2 h), and MAFP (an ExoU inhibitor) caused a dose-dependent reduction of this effect, implying that these cytotoxicities were due to the effector ExoU. On the other hand, P206 and P231 caused loss of Vero cell viability only after prolonged incubation periods (10 h) and were unaffected by both MAFP and E216-5138 (ExoS inhibitor), indicating that ExoT may be the effector responsible for the observed cytotoxic effects. Several compounds were tested to inhibit bacterial cytotoxicity. Mannose of the tested antiadherents and poloxamer 407 of the tested .

• Sinh học
• Pseudomonas aeruginosa
• Vero cells
• Type III secretion system
• G protein activating protein
• ADP ribosylation
• Đồ án tốt nghiệp đại học
• Công nghệ sinh học
• Độ nhiễm khuẩn của Pseudomonas aeruginosa
• Khả năng kháng kháng sinh của Pseudomonas aeruginosa
• Nhiễm khuẩn nước uống
• Định lượng Pseudomonas aeruginosa trong nước uống
• Kháng kháng sinh
• Vi khuẩn từ bệnh phẩm
• Chủng P
• aeruginosa
• Bệnh nhân nhiễm Pseudomonas aeruginosa
• Sản xuất Carbapenemase
• Metallo beta lactamase
• Producing pseudomonas Aeruginosa isolates
• Aeruginosa isolates
• Multidrug resistance
• Pseudomonas aeruginosa (P aeruginosa)
• Antimicrobial resistance pattern
• Various clinical samples
• Government medical college
• Đề kháng kháng sinh Staphylococci
• Đề kháng kháng sinh Pseudomonas aeruginosa
• Viêm loét giác mạc
• Bệnh viện mắt thành phố Hồ Chí Minh
• Đề kháng kháng sinh thông dụng
• Antimicrobial sensitivity testing
• Drug resistance
• Medical tips
• Blood from patients
• Pseudomonas aeruginosa recovered
• LasA protease
• LasB protease
• Biotechnological potential
• Non pathogenic environmental isolate
• Pseudomonas aeruginosa MCCB 123
• Extracellular polysaccharides
• Larvicidal activity
• Pseudomonas aeruginosa B01
• Isolation and characterization
• Larvicidal extracellular polysaccharide
• Clinical isolates
• OprD protein profile
• Pseudomonas Aeruginosa isolates resistant
• Imipenem from patients
• Sludge using Pseudomonas Aeruginosa
• Environmental maintenance
• Industrial waste management
• Heavy metal like LEAD
• Celosia Cristata L
• Celosia cristata
• Pseudomonas Aeruginosa MCC 3198
• Potential for growth promotion
• Medicinal plant Celosia Cristata L
• Degradation of discarded
• Engine oil by Pseudomonas aeruginosa DP 1
• Used engine oil
• Oil degrading organisms
• Combined disc test
• MBL producing Pseudomonas aeruginosa
• Prevalence of ESBL
• Tertiary care hospital
• Nanosilver and antibiotics
• Antibiotics separately
• Pseudomonas aeruginosa clinical isolates
• Silver nano particles may provide safe
• Carbapenem resistance
• MBL genes
• Characterization of VIM
• IMP Metallobetalactamases
• Pseudomonas aeruginosa isolated
• Various clinical specimens
• Antibiotic resistance
• Pseudomonas aeruginosa strains isolated
• Skin host defences are destroyed
• Antibacterial activity
• Broccoli extracts
• Against Pseudomonas aeruginosa
• Antibacterial potential
• Kaempheria galangal
• Anti biofilm
• Anti plasmid pseudomonas aeruginosa
• Anti plasmid activites
• OprD Deficient Mutant
• Pseudomonas aeruginosa carbapenemase
• New phenotypic method
• Screen for OprD deficient mutant strains
• Extended spectrum beta lactamase
• Non extended spectrum beta lactamase
• Producing pseudomonas aeruginosa
• Occurrence of antimicrobial resistance
• Nosocomial infection
• Quantitative biofilm assay
• Pseudomonas aeruginosa isolates
• Clinical samples
• Arginine deaminase
• Fermentation parameters
• Bacterium pseudomonas aeruginosa PS2
• Arginine deaminase production
• Công nghệ hóa
• Khả năng hấp thu toluen
• Pseudomonas putida
• Hóa hữu cơ
• Ô nhiễm môi trường
• PCR based detection
• Mastitic cow milk
• Bovine Mastitis