TAILIEUCHUNG - Production of high titer of citric acid from inulin

Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. | Rakicka et al. BMC Biotechnology 2019 19 11 https s12896-019-0503-0 BMC Biotechnology RESEARCH ARTICLE Open Access Production of high titer of citric acid from inulin Magdalena Rakicka Jakub Wolniak Zbigniew Lazar and Waldemar Rymowicz Abstract Background Citric acid is considered as the most economically feasible product of microbiological production therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study citric acid was synthesized by genetically engineered strains of Yarrowia lipolytica from widely available renewable polysaccharide - inulin. Hydrolysis of inulin by the Y. lipolytica strains was established by expressing the inulinase gene INU1 gene GenBank with its native secretion signal sequence was amplified from genomic DNA from Kluyveromyces marxianus CBS6432. To ensure the maximum citric acid titer the optimal cultivation strategy-repeated-batch culture was applied. Results The strain Y. lipolytica AWG7 INU 8 secreted more than 200 g dm-3 of citric acid during repeated-batch culture on inulin with a productivity of g dm-3 h-1 and a yield of g g-1. Conclusions The citric acid titer obtained in the proposed process is the highest value reported in the literature for Yarrowia yeast. The obtained results suggest that citric acid production from inulin by engineered Y. lipolytica may be a very promising technology for industrial citric acid production. Keywords Inulin Citric acid Repeated-batch culture Yarrowia lipolytica Background Production of citric acid CA the intermediate of Krebs cycle is one of the oldest technology of organic acid production applied at industrial scale 1 . Initially CA was extracted from Italian lemons which what was later replaced by its biosynthesis using Aspergillus niger 2 . Afterwards the discovery of CA secretion by the yeast Yarrowia lipolytica caused a rapid progress in research on that process 3 . The global CA production in 2015 reached

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