TAILIEUCHUNG - Evaluation of different methods for conversion of whole virion particle (146S) of FMDV into 12s subunits and application in characterization of monoclonal antibodies

Foot-and-mouth disease (FMD) is a highly contagious disease of cloven hoofed animals caused by FMD virus classified under genus Apthovirus and Picornaviridae family. Monoclonal antibodies (mAb) to FMDV provides best tool for the development of reliable diagnostics and there is a need for the development of mAbs that specifically recognize either 146S or 12S for the quality control analysis of FMDV vaccines. In the present study, we developed mAbs specific to FMDV serotype O and characterized their specificity in recognition of 146S or 12S particles. Three different methods were evaluated for the conversion of 146S into 12S subunits that includes heat method, strong acid and mild acid methods for testing the reactivity of mAbs with these antigens by double antibody sandwich ELISA. Mild heating of 146S antigens at 560C for 1hour and treatment of 146S antigen with weak acid ( NaH2PO4, pH - ) resulted in complete conversion of 146S into 12S. Where as in strong acid method (1N HCl with pH below ), 146S treated with 1N HCl resulted in loss of antigenicity of 12S subunits that reflected in absence of reactivity in ELISA. This may mislead to consider the mAb as 146S specific. This study revealed that mAb binding epitopes of all monoclonals are commonly shared among 146S and 12S antigens. Further it helps in understanding the specificity of mAbs to 146S and 12S particles which will be useful for application of mAbs in different diagnostic assays. | Evaluation of different methods for conversion of whole virion particle (146S) of FMDV into 12s subunits and application in characterization of monoclonal antibodies

• Sinh học
• Foot and mouth disease virus
• Monoclonal antibodies
• Whole virion particle
• 12S subunits
• Monoclonal antibodies
• Chromatography A
• Ion exchange
• Downstream processing
• BMC Biotechnology
• Humanized rats
• Human antibodies
• Human idiotypes
• Recombinant antibodies
• Mammalian cell based screening mammalian cell culture
• Protein production
• Ammonium sulfate
• Multiplex analysis
• Therapeutic monoclonal antibody
• Hybridomas
• Interleukin 6
• Supplemental media
• Kháng thể đơn dòng
• Sản xuất kháng thể đơn dòng
• Monoclonal antibodies Mab
• Kháng thể đa dòng
• 8 bước tạo MAb
• Tinh chế MAb
• Monoclonal antibodies specific
• Serum progesterone
• Hybrid cells
• Hybridoma technology
• Enzyme linked immunosorbent assay
• Virus like particles
• Porcine circovirus 2
• PCV2 associated diseases
• BMC Cancer
• Meta analysis
• Multiple myeloma
• Randomised controlled trials
• Rabbit anti mouse serum albumin polyclonal antibody generation
• Release testing of monoclonal antibodies
• Murine ascites fluid
• Mouse serum albumin
• Polyclonal antibody
• Protein A affinity chromatography
• Host cell proteins
• Downstream bioprocessing
• Antibody genes
• B cell receptors
• Comparative efficacy
• Different methods
• Generation of 12S particles
• 146S particles of FMDV serotype
• Specific monoclonal antibodies
• Dengue virus
• NS1 protein
• Mouse immunization
• HEK cells
• Monoclonal antibody
• Serum free medium
• Gel filtration chromotography
• Tobacco mosaic virus
• Hybrid cell
• Steroid hormones
• Serum albumine
• Chinese hamster ovary
• Epigenetic gene silencing
• Chromatin opening element
• Sodium butyrate
• Valproic acid
• C terminal amidation
• Genetic modification
• Recombinant monoclonal antibody
• Peptidylglycine α amidating monooxygenase
• BMC Developmental Biology
• Monoclonal antibody screen
• Immunological resources
• Molecular mechanisms
• Schmidtea mediterranea
• Breast cancer
• Ovarian cancer
• Ovarian cancer patients
• Quantify serum CA125 concentration
• Superficially porous particles
• Low hydrophobicity
• Reversed phase liquid chromatography
• Ion exchange chromatography
• Mab biosimilars
• Cation exchange chromatography
• Continuous chromatography
• Process development
• Protein A
• Integrated continuous downstream process
• Mild purification
• Process design